|
| Status |
Public on Feb 13, 2013 |
| Title |
MM_#42R_blood |
| Sample type |
genomic |
| |
|
| Source name |
Bone marrow and/or blood
|
| Organism |
Homo sapiens |
| Characteristics |
patient id: MM_#42
tumor: multiple myeloma
tissue: blood
cells: purified CD138+ plasma cells
sample type: Relapse
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was extracted using the DNA AllPrep DNA/RNA MiniKit (Qiagen, Valencia, CA). Matched germline DNA from 17 patients was extracted from buffy coat and purified using Nucleon BACC Genomic DNA Extraction Kit (GE Healthcare Life Sciences Piscataway, NJ). DNA quality and quantity were assessed using the Nanodrop Spectrophotometer (NanoDrop Technologies, Inc. Wilmington, DE).
|
| Label |
Biotin
|
| Label protocol |
According to manufacturer protocol
|
| |
|
| Hybridization protocol |
DNA was restriction digested, PCR amplified, fragmented, labeled and hybridized to each array according to the manufacturer's instructions.
|
| Scan protocol |
Arrays were scanned using the GeneChip Scanner 3000 7G.
|
| Description |
Blood
|
| Data processing |
Affymetrix Genotyping Console 4.0 for processing SNPs, detecting copy number abnormalities with default parameters and 270 HapMap reference samples.
|
| |
|
| Submission date |
Nov 10, 2010 |
| Last update date |
Feb 13, 2013 |
| Contact name |
Wilfried Gouraud |
| Organization name |
ICO - UMGC
|
| Department |
Integrated Center of Oncology René Gauducheau
|
| Lab |
Omics Data Science Unit
|
| Street address |
bd Jacques Monod
|
| City |
Saint Herblain |
| ZIP/Postal code |
44805 |
| Country |
France |
| |
|
| Platform ID |
GPL6801 |
| Series (2) |
| GSE25262 |
Genetic adaptability of cancer cells under treatment selection pressure in multiple myeloma patients. |
| GSE37469 |
Minor clone provides a reservoir for relapse in multiple myeloma |
|
