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Status |
Public on Sep 15, 2023 |
Title |
Vehicle treated ICH mice, Day 5_3 |
Sample type |
SRA |
|
|
Source name |
perihematomal brain tissue
|
Organism |
Mus musculus |
Characteristics |
tissue: perihematomal brain tissue cell type: WT treatment: Vehicle time point: Day 5
|
Treatment protocol |
Mice were treated with TEPP-46 or vehicle for 5 consective days after ICH.
|
Extracted molecule |
total RNA |
Extraction protocol |
perihematomal brain tissues were removed, flash frozen on dry ice, and total RNA was extracted using TRIzol reagent (Takara, Kyoto, Japan) according to the manufacturer’s instructions cDNA libraries were constructed from 1 μg of total RNA by using a cDNA-PCR Sequencing Kit (SQK-PCS109) according to the protocol.
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|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
PromethION |
|
|
Data processing |
Raw reads were first filtered with minimum average read quality score=7 and minimum read length=500bp. full-length, non-chemiric (FLNC) transcripts were determined by searching for primer at both ends of reads. Clusters of FLNC transcripts were obtained after mapping to reference genome with mimimap2. Differential expression analysis of two conditions/groups was performed using the DESeq R package (1.10.1). Assembly: GRCm38 Supplementary files format and content: gene expression of all sample
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Submission date |
May 30, 2022 |
Last update date |
Sep 15, 2023 |
Contact name |
Xiao-Yi Xiong |
E-mail(s) |
xiongxy1989@hotmail.com
|
Organization name |
Chengdu University of Traditional Chinese Medicine
|
Street address |
37 Shierqiao Road, 610075, Chengdu, China
|
City |
Chengdu |
ZIP/Postal code |
610075 |
Country |
China |
|
|
Platform ID |
GPL26624 |
Series (1) |
GSE205082 |
Expression profiles of perihematomal brain tissues of intracerebral hemorrhage (ICH) mice treated with or without TEPP-46 |
|
Relations |
BioSample |
SAMN28766655 |
SRA |
SRX15499128 |