|
Status |
Public on Oct 31, 2023 |
Title |
Brd9_KO_03 |
Sample type |
SRA |
|
|
Source name |
Lineage- bone marrow cells in femora and tibiae
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6 genotype: CD45.2(+) Mx1-Cre Brd9fl/fl timing of preparation: Eight weeks after pIpC injection
|
Treatment protocol |
Four weeks after BMT, the recipient mice received 20 mg/kg pIpC injection every other day for a total of 3 doses.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted using RNeasy columns (Qiagen) per manufacturer’s instructions. RNA was poly(A)-selected, and unstranded Illumina libraries were prepared with the standard TruSeq protocol
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Data processing |
Raw FASTQs were processed using nf-core/rnaseq (3.0). Assembly: mm10 Supplementary files format and content: txt
|
|
|
Submission date |
May 18, 2022 |
Last update date |
Oct 31, 2023 |
Contact name |
Masaki Nomura |
E-mail(s) |
masaki.nomura.fbri@gmail.com
|
Organization name |
Foundation for Biomedical Research and Innovation at Kobe
|
Street address |
6-3-7 Minatojima Minamimachi, Chuo Ward
|
City |
Kobe |
State/province |
Hyogo |
ZIP/Postal code |
650-0047 |
Country |
Japan |
|
|
Platform ID |
GPL24247 |
Series (2) |
GSE203316 |
BRD9 determines the cell fate of hematopoietic stem cells by regulating chromatin state [bulk RNA-Seq I] |
GSE203322 |
BRD9 determines the cell fate of hematopoietic stem cells by regulating chromatin state |
|
Relations |
BioSample |
SAMN28534445 |
SRA |
SRX15336991 |