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Sample GSM6165222 Query DataSets for GSM6165222
Status Public on May 15, 2024
Title AHC [4549ADC212]
Sample type RNA
 
Source name PBMC from AHC
Organism Homo sapiens
Characteristics gender: Male
age (years): 38
Treatment protocol PBMC were purified from Ficoll hypaque gradient (Invitrogen, USA)
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description PBMC gene expression from AHC patients
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Following scanning of the arrays, the microarray raw data were initially normalized by R Bioconductor Packages, all arrays were normalized serially using a single sample microarray normalization approach designed for personal medicine workflows (SCAN Normalization). An empirical Bayes approach (ComBat algorithm) was used to minimize the batch effect in the data
 
Submission date May 18, 2022
Last update date May 15, 2024
Contact name Michael Gurevich
E-mail(s) Michael.Gurevich@sheba.health.gov.il
Organization name Sheba Medical Center
Department Multiple Sclerosis Center
Lab Neurogenomics and Neuroimmunology
Street address Tel Hashomer
City Ramat Gan
ZIP/Postal code 52621
Country Israel
 
Platform ID GPL571
Series (1)
GSE203241 Age-related blood transcriptional regulators affect disease progression in pediatric multiple sclerosis

Data table header descriptions
ID_REF
VALUE normalized signal

Data table
ID_REF VALUE
1007_s_at 0.443689
1053_at 0.384013
117_at 0.761304
121_at 0.401519
1255_g_at -0.178282
1294_at 1.17382
1316_at 0.236307
1320_at -0.15199
1405_i_at 2.48948
1431_at -0.0981692
1438_at -0.160255
1487_at 0.73624
1494_f_at -0.030493
1598_g_at 0.292852
160020_at 0.50328
1729_at 1.217
177_at -0.0694072
1773_at -0.0244206
179_at 0.437032
1861_at -0.128975

Total number of rows: 22277

Table truncated, full table size 438 Kbytes.




Supplementary file Size Download File type/resource
GSM6165222_4549ADC212.CEL.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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