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Sample GSM6165211 Query DataSets for GSM6165211
Status Public on May 15, 2024
Title PHC [6303PDC210_(HG-U133A_2)]
Sample type RNA
Source name PBMC from PHC
Organism Homo sapiens
Characteristics gender: Male
age (years): 12
Treatment protocol PBMC were purified from Ficoll hypaque gradient (Invitrogen, USA)
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description PBMC gene expression from PHC patients
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Following scanning of the arrays, the microarray raw data were initially normalized by R Bioconductor Packages, all arrays were normalized serially using a single sample microarray normalization approach designed for personal medicine workflows (SCAN Normalization). An empirical Bayes approach (ComBat algorithm) was used to minimize the batch effect in the data
Submission date May 18, 2022
Last update date May 15, 2024
Contact name Michael Gurevich
Organization name Sheba Medical Center
Department Multiple Sclerosis Center
Lab Neurogenomics and Neuroimmunology
Street address Tel Hashomer
City Ramat Gan
ZIP/Postal code 52621
Country Israel
Platform ID GPL571
Series (1)
GSE203241 Age-related blood transcriptional regulators affect disease progression in pediatric multiple sclerosis

Data table header descriptions
VALUE normalized signal

Data table
1007_s_at 0.38432
1053_at 0.381124
117_at 0.365154
121_at 0.379267
1255_g_at -0.164646
1294_at 0.791734
1316_at 0.402402
1320_at -0.176714
1405_i_at 2.53852
1431_at -0.147981
1438_at -0.113883
1487_at 0.666483
1494_f_at -0.0860001
1598_g_at 0.225657
160020_at 0.50389
1729_at 0.785137
177_at -0.0939004
1773_at 0.0163416
179_at 0.417412
1861_at 0.162419

Total number of rows: 22277

Table truncated, full table size 440 Kbytes.

Supplementary file Size Download File type/resource
GSM6165211_6303PDC210_HG-U133A_2_.CEL.gz 1.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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