NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM605925 Query DataSets for GSM605925
Status Public on Oct 08, 2010
Title BT549
Sample type RNA
 
Source name Cultured cell line
Organism Homo sapiens
Characteristics cell type: breast cancer cell
cell line: BT549
Treatment protocol No treatment
Growth protocol Subconfuent breast cancer cell lines
Extracted molecule total RNA
Extraction protocol RNeasy mini kit (Qiagen)
Label biotin
Label protocol Biotinylated cRNA were prepared using Ambion MessageAmp Premier labeling kit.
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133A plus 2.0 arrays. Affymetrix GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix Scanner 3000 7G.
Description Gene expression data from breast cancer cell lines
Data processing The data were analyzed with RMA (Robust Multichip Average) using Bioconductor
 
Submission date Oct 07, 2010
Last update date Oct 07, 2010
Contact name Igor Shats
E-mail(s) is37@duke.edu
Organization name Duke University
Street address 101 Science Drive
City Durham
State/province NC
ZIP/Postal code 27710
Country USA
 
Platform ID GPL571
Series (2)
GSE24578 Basal gene expression of breast cancer cell lines
GSE24717 Using a Stem Cell-Based Signature to Guide Therapeutic Selection in Cancer

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
1007_s_at 8.482082537
1053_at 9.49941079
117_at 5.774157355
121_at 8.53496028
1255_g_at 3.423670696
1294_at 5.603432937
1316_at 6.435922448
1320_at 5.259933494
1405_i_at 6.246836772
1431_at 4.098625954
1438_at 6.089886641
1487_at 8.701618427
1494_f_at 6.342169808
1598_g_at 10.71527593
160020_at 8.632296951
1729_at 8.692757017
177_at 5.570511689
1773_at 6.572547943
179_at 8.994577798
1861_at 8.160485804

Total number of rows: 22277

Table truncated, full table size 496 Kbytes.




Supplementary file Size Download File type/resource
GSM605925.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap