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Sample GSM605922 Query DataSets for GSM605922
Status Public on Oct 08, 2010
Title T47D
Sample type RNA
 
Source name Cultured cell line
Organism Homo sapiens
Characteristics cell type: breast cancer cell
cell line: T47D
Treatment protocol No treatment
Growth protocol Subconfuent breast cancer cell lines
Extracted molecule total RNA
Extraction protocol RNeasy mini kit (Qiagen)
Label biotin
Label protocol Biotinylated cRNA were prepared using Ambion MessageAmp Premier labeling kit.
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133A plus 2.0 arrays. Affymetrix GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix Scanner 3000 7G.
Description Gene expression data from breast cancer cell lines
Data processing The data were analyzed with RMA (Robust Multichip Average) using Bioconductor
 
Submission date Oct 07, 2010
Last update date Oct 07, 2010
Contact name Igor Shats
E-mail(s) is37@duke.edu
Organization name Duke University
Street address 101 Science Drive
City Durham
State/province NC
ZIP/Postal code 27710
Country USA
 
Platform ID GPL571
Series (2)
GSE24578 Basal gene expression of breast cancer cell lines
GSE24717 Using a Stem Cell-Based Signature to Guide Therapeutic Selection in Cancer

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
1007_s_at 11.68479483
1053_at 9.646240479
117_at 5.586291992
121_at 8.103863776
1255_g_at 3.399451278
1294_at 5.756002902
1316_at 6.92079553
1320_at 6.049760574
1405_i_at 5.011171665
1431_at 4.050880993
1438_at 8.075676833
1487_at 8.695292096
1494_f_at 6.203945322
1598_g_at 10.3426301
160020_at 7.019455837
1729_at 8.698236108
177_at 5.941806425
1773_at 7.048966419
179_at 9.168930796
1861_at 8.493269138

Total number of rows: 22277

Table truncated, full table size 496 Kbytes.




Supplementary file Size Download File type/resource
GSM605922.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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