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Sample GSM605920 Query DataSets for GSM605920
Status Public on Oct 08, 2010
Title MCF7
Sample type RNA
 
Source name Cultured cell line
Organism Homo sapiens
Characteristics cell type: breast cancer cell
cell line: MCF7
Treatment protocol No treatment
Growth protocol Subconfuent breast cancer cell lines
Extracted molecule total RNA
Extraction protocol RNeasy mini kit (Qiagen)
Label biotin
Label protocol Biotinylated cRNA were prepared using Ambion MessageAmp Premier labeling kit.
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133A plus 2.0 arrays. Affymetrix GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix Scanner 3000 7G.
Description Gene expression data from breast cancer cell lines
Data processing The data were analyzed with RMA (Robust Multichip Average) using Bioconductor
 
Submission date Oct 07, 2010
Last update date Oct 07, 2010
Contact name Igor Shats
E-mail(s) is37@duke.edu
Organization name Duke University
Street address 101 Science Drive
City Durham
State/province NC
ZIP/Postal code 27710
Country USA
 
Platform ID GPL571
Series (2)
GSE24578 Basal gene expression of breast cancer cell lines
GSE24717 Using a Stem Cell-Based Signature to Guide Therapeutic Selection in Cancer

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
1007_s_at 11.41438128
1053_at 9.187854479
117_at 5.769059225
121_at 8.162400381
1255_g_at 3.189836623
1294_at 5.615288191
1316_at 6.012564136
1320_at 5.20021417
1405_i_at 5.308657283
1431_at 3.983739193
1438_at 7.288233727
1487_at 8.762163771
1494_f_at 6.149291495
1598_g_at 8.721762653
160020_at 6.912200551
1729_at 8.866341397
177_at 5.202728516
1773_at 7.134561769
179_at 9.100319987
1861_at 7.792978659

Total number of rows: 22277

Table truncated, full table size 496 Kbytes.




Supplementary file Size Download File type/resource
GSM605920.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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