NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM605918 Query DataSets for GSM605918
Status Public on Oct 08, 2010
Title SKBR3
Sample type RNA
 
Source name Cultured cell line
Organism Homo sapiens
Characteristics cell type: breast cancer cell
cell line: SKBR3
Treatment protocol No treatment
Growth protocol Subconfuent breast cancer cell lines
Extracted molecule total RNA
Extraction protocol RNeasy mini kit (Qiagen)
Label biotin
Label protocol Biotinylated cRNA were prepared using Ambion MessageAmp Premier labeling kit.
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133A plus 2.0 arrays. Affymetrix GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix Scanner 3000 7G.
Description Gene expression data from breast cancer cell lines
Data processing The data were analyzed with RMA (Robust Multichip Average) using Bioconductor
 
Submission date Oct 07, 2010
Last update date Oct 07, 2010
Contact name Igor Shats
E-mail(s) is37@duke.edu
Organization name Duke University
Street address 101 Science Drive
City Durham
State/province NC
ZIP/Postal code 27710
Country USA
 
Platform ID GPL571
Series (2)
GSE24578 Basal gene expression of breast cancer cell lines
GSE24717 Using a Stem Cell-Based Signature to Guide Therapeutic Selection in Cancer

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
1007_s_at 11.68487806
1053_at 9.887069951
117_at 5.750795955
121_at 7.996422282
1255_g_at 3.317067914
1294_at 6.44992801
1316_at 7.045476463
1320_at 6.298481998
1405_i_at 7.205356669
1431_at 4.082561705
1438_at 8.110600007
1487_at 9.770982682
1494_f_at 6.029989481
1598_g_at 8.087279377
160020_at 6.65534419
1729_at 8.249751871
177_at 5.71745908
1773_at 7.23039158
179_at 8.928369222
1861_at 8.215956571

Total number of rows: 22277

Table truncated, full table size 496 Kbytes.




Supplementary file Size Download File type/resource
GSM605918.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap