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Sample GSM605916 Query DataSets for GSM605916
Status Public on Oct 08, 2010
Title MDA-MB-231
Sample type RNA
Source name Cultured cell line
Organism Homo sapiens
Characteristics cell type: breast cancer cell
cell line: MDA-MB-231
Treatment protocol No treatment
Growth protocol Subconfuent breast cancer cell lines
Extracted molecule total RNA
Extraction protocol RNeasy mini kit (Qiagen)
Label biotin
Label protocol Biotinylated cRNA were prepared using Ambion MessageAmp Premier labeling kit.
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133A plus 2.0 arrays. Affymetrix GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix Scanner 3000 7G.
Description Gene expression data from breast cancer cell lines
Data processing The data were analyzed with RMA (Robust Multichip Average) using Bioconductor
Submission date Oct 07, 2010
Last update date Oct 07, 2010
Contact name Igor Shats
Organization name Duke University
Street address 101 Science Drive
City Durham
State/province NC
ZIP/Postal code 27710
Country USA
Platform ID GPL571
Series (2)
GSE24578 Basal gene expression of breast cancer cell lines
GSE24717 Using a Stem Cell-Based Signature to Guide Therapeutic Selection in Cancer

Data table header descriptions

Data table
1007_s_at 7.9066689
1053_at 9.063946826
117_at 5.740153993
121_at 8.560396702
1255_g_at 3.188509508
1294_at 5.996741501
1316_at 6.103781161
1320_at 5.294703617
1405_i_at 4.523025007
1431_at 4.23353961
1438_at 6.029734834
1487_at 8.792279046
1494_f_at 6.259393313
1598_g_at 10.44643701
160020_at 8.924209544
1729_at 8.466015082
177_at 5.198956105
1773_at 6.521027726
179_at 8.741074094
1861_at 8.447263197

Total number of rows: 22277

Table truncated, full table size 496 Kbytes.

Supplementary file Size Download File type/resource
GSM605916.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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