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Sample GSM605912 Query DataSets for GSM605912
Status Public on Oct 08, 2010
Title BT483
Sample type RNA
Source name Cultured cell line
Organism Homo sapiens
Characteristics cell type: breast cancer cell
cell line: BT483
Treatment protocol No treatment
Growth protocol Subconfuent breast cancer cell lines
Extracted molecule total RNA
Extraction protocol RNeasy mini kit (Qiagen)
Label biotin
Label protocol Biotinylated cRNA were prepared using Ambion MessageAmp Premier labeling kit.
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133A plus 2.0 arrays. Affymetrix GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix Scanner 3000 7G.
Description Gene expression data from breast cancer cell lines
Data processing The data were analyzed with RMA (Robust Multichip Average) using Bioconductor
Submission date Oct 07, 2010
Last update date Oct 07, 2010
Contact name Igor Shats
Organization name Duke University
Street address 101 Science Drive
City Durham
State/province NC
ZIP/Postal code 27710
Country USA
Platform ID GPL571
Series (2)
GSE24578 Basal gene expression of breast cancer cell lines
GSE24717 Using a Stem Cell-Based Signature to Guide Therapeutic Selection in Cancer

Data table header descriptions

Data table
1007_s_at 12.01264361
1053_at 8.602476048
117_at 5.748341097
121_at 7.905451625
1255_g_at 3.242775338
1294_at 5.691093657
1316_at 7.154965833
1320_at 5.014877159
1405_i_at 5.77383555
1431_at 5.105952524
1438_at 7.480833497
1487_at 9.04744826
1494_f_at 6.206143155
1598_g_at 9.920439874
160020_at 6.800923126
1729_at 8.44160941
177_at 5.292256075
1773_at 7.302543018
179_at 8.884265851
1861_at 8.261663489

Total number of rows: 22277

Table truncated, full table size 496 Kbytes.

Supplementary file Size Download File type/resource
GSM605912.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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