|
Status |
Public on Jan 20, 2012 |
Title |
Tumor from ApcD14/+:Arrb2+/+ mouse 6 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
Tumor from ApcD14/+:Arrb2+/+ mouse 6
|
Organism |
Mus musculus |
Characteristics |
strain: C57Bl/6 mouse: 6 genotype/variation: ApcD14/+:Arrb2+/+ tissue: tumor sample type: Test
|
Growth protocol |
Tumors kept in RNA later at -80°C before RNA extraction
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extracted by RNable according to manufacturer's instructions
|
Label |
Cy5
|
Label protocol |
For the preparation of the labelled Cy3- and Cy5- aRNA target, one microgram of total RNA samples were amplified and labelled using Amino Allyl Message Amp II aRNA amplification kit (Ambion, Austin, TX, USA), according the manufacturer’s instructions.
|
|
|
Channel 2 |
Source name |
Pool of equimolar RNA of each sample
|
Organism |
Mus musculus |
Characteristics |
strain: C57Bl/6 sample type: Reference
|
Growth protocol |
Tumors kept in RNA later at -80°C before RNA extraction
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extracted by RNable according to manufacturer's instructions
|
Label |
Cy3
|
Label protocol |
For the preparation of the labelled Cy3- and Cy5- aRNA target, one microgram of total RNA samples were amplified and labelled using Amino Allyl Message Amp II aRNA amplification kit (Ambion, Austin, TX, USA), according the manufacturer’s instructions.
|
|
|
|
Hybridization protocol |
The two labelled aRNA were mixed, fragmented and hybridized on one array of a Nimblegen 12-plex microarray according the manufacturer's instructions.
|
Scan protocol |
Scanned on an Innopsys Innoscan900 scanner.
|
Description |
ApcD14-6
|
Data processing |
Images were quantified using Nimblescan V2.5. The rawdata were normalized by Lowess and a quantile normalization was applied (Limma Package).
|
|
|
Submission date |
Oct 07, 2010 |
Last update date |
Jan 20, 2012 |
Contact name |
Caroline Bonnans |
E-mail(s) |
cbonnans@univ-montp1.fr
|
Phone |
+33-4-67-66-81-60
|
Organization name |
Functional Genomic Institute
|
Department |
Oncology
|
Street address |
141 rue de la Cardonille
|
City |
Montpellier |
ZIP/Postal code |
34094 |
Country |
France |
|
|
Platform ID |
GPL10994 |
Series (2) |
GSE24575 |
beta-arrestin-2 deficiency effect on intestinal tumorigenesis in Apc-mutated mice |
GSE24577 |
Impact of beta-arrestin-2 deficiency on intestinal tumorigenesis in Apc-mutated mice |
|