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Sample GSM6049796 Query DataSets for GSM6049796
Status Public on Apr 16, 2023
Title murineCB_Myd88L252P_repl3
Sample type SRA
 
Source name Centroblast
Organism Mus musculus
Characteristics sorting: B220+FAS+GL7+CXCR4+CD86-
genotype: Cgamma1Cre: +/-; Myd88L252P: +/-
Extracted molecule total RNA
Extraction protocol RNA-seq libraries were prepared using the Illumina TruSeq stranded mRNA sample kits, respectively, according to the manufacturer. Libraries were validated using the Agilent Technologies 2100 Bioanalyzer and Quant-iTâ„¢ dsDNA HS Assay (Life Technologies)
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NextSeq 500
 
Data processing Illumina Casava1.8 software used for basecalling.
RNA-seq reads were aligned to mm10 using STAR with default parameters
TPM were obtained using Rsubread and RefSeq gene annotation
Assembly: mm10
Supplementary files format and content: tab-delimited
 
Submission date Apr 19, 2022
Last update date Apr 17, 2023
Contact name Matt Teater
E-mail(s) mrt2001@med.cornell.edu
Organization name Weill Cornell Medical College
Street address 445 E 69th St
City New York
State/province NY
ZIP/Postal code 10021
Country USA
 
Platform ID GPL19057
Series (1)
GSE201058 Transcriptional profiling of Myd88 L252P germinal center B-cells
Relations
BioSample SAMN27642001
SRA SRX14918040

Supplementary file Size Download File type/resource
GSM6049796_Sample_8_murineCB_Myd88L252P_repl3_TPM.txt.gz 216.3 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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