|
Status |
Public on Apr 16, 2023 |
Title |
murineCB_Control_repl3 |
Sample type |
SRA |
|
|
Source name |
Centroblast
|
Organism |
Mus musculus |
Characteristics |
sorting: B220+FAS+GL7+CXCR4+CD86- genotype: Cgamma1Cre: +/-; Myd88L252P: WT
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA-seq libraries were prepared using the Illumina TruSeq stranded mRNA sample kits, respectively, according to the manufacturer. Libraries were validated using the Agilent Technologies 2100 Bioanalyzer and Quant-iTâ„¢ dsDNA HS Assay (Life Technologies)
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NextSeq 500 |
|
|
Data processing |
Illumina Casava1.8 software used for basecalling. RNA-seq reads were aligned to mm10 using STAR with default parameters TPM were obtained using Rsubread and RefSeq gene annotation Assembly: mm10 Supplementary files format and content: tab-delimited
|
|
|
Submission date |
Apr 19, 2022 |
Last update date |
Apr 17, 2023 |
Contact name |
Matt Teater |
E-mail(s) |
mrt2001@med.cornell.edu
|
Organization name |
Weill Cornell Medical College
|
Street address |
445 E 69th St
|
City |
New York |
State/province |
NY |
ZIP/Postal code |
10021 |
Country |
USA |
|
|
Platform ID |
GPL19057 |
Series (1) |
GSE201058 |
Transcriptional profiling of Myd88 L252P germinal center B-cells |
|
Relations |
BioSample |
SAMN27642006 |
SRA |
SRX14918035 |