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Sample GSM595436 Query DataSets for GSM595436
Status Public on Nov 18, 2010
Title Mouse heart, biological replicate 3
Sample type RNA
 
Source name Mouse heart
Organism Mus musculus
Characteristics tissue: heart
strain: C57Bl6
Sex: male
age: 10-12 weeks old
Extracted molecule total RNA
Extraction protocol Total RNA from mouse tussues was extracted using Trizol reagent according to the manufacturer's protocol (Invitrogen), followed by a cleanup procedure with Rneasy colmuns (Qiagen). RNA from mouse islets as well as mouse pituitary and adrenal gland was extracted using the absolutely RNA microprep (Stratagene). For MIN6 cells we used the PureLink micro-to-midi RNA kit (INvitrogen)
Label Biotin
Label protocol Cellular mRNA was reverse transcribed into cDNA (SuperScript Choice System Invitrogen, Carlsbad, CA using oligo-dT primers and a T7 RNA polymerase promoter site). Two ug of total RNA was used to prepare biotinylated cRNA with IVT labeling kit (Affymetrix, Santa Clara, CA) according to the Genechip expression analysis technical manual 701025 Rev.5. The concentration of labelled cRNA was measured using the NanoDrop ND-1000 spectrophotometer. Labeled cRNA was fragmented in a fragmentation buffer during 35 min at 94C. The quality of labelled and fragmented cRNA was analysed using the Agilent bioanalyzer 2100.
 
Hybridization protocol Fragmented cRNA was hybridised to mouse 430 2.0 arrays (Affymetrix) during 16h at 45C.
Scan protocol The arrays were washed and stained in a fluidics station (Affymetrix) and scanned using the Affymetrix 3000 GeneScanner.
Description CEL file uploaded in GSE9954
GSM252115
Data processing All images were analyzed using GCOS version 1.4 with the MAS5 algorithm
 
Submission date Sep 17, 2010
Last update date Nov 18, 2010
Contact name Katleen Lemaire
E-mail(s) katleen.lemaire@med.kuleuven.be
Organization name K.U.Leuven
Street address Herestraat 49, bus 901
City Leuven
ZIP/Postal code 3000
Country Belgium
 
Platform ID GPL1261
Series (1)
GSE24207 mRNA analysis in different mouse tissues

Data table header descriptions
ID_REF
VALUE absolute MAS5 signals

Data table
ID_REF VALUE
1415670_at 264
1415671_at 652
1415672_at 1340
1415673_at 106
1415674_a_at 454
1415675_at 145
1415676_a_at 1359
1415677_at 305
1415678_at 1123
1415679_at 973
1415680_at 169
1415681_at 665
1415682_at 167
1415683_at 879
1415684_at 210
1415685_at 513
1415686_at 457
1415687_a_at 3463
1415688_at 1542
1415689_s_at 216

Total number of rows: 45037

Table truncated, full table size 642 Kbytes.




Supplementary file Size Download File type/resource
GSM595436.CEL.gz 3.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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