|
| Status |
Public on Dec 22, 2010 |
| Title |
AML_LSC_TLA_SU032 |
| Sample type |
RNA |
| |
|
| Source name |
therapeutic leukapheresis
|
| Organism |
Homo sapiens |
| Characteristics |
patient: SU032
age group: adult
disease state: AML
cell source: therapeutic leukapheresis
cell type (purified cell subset): Lin-CD34+CD38-CD90-
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using TRIzol reagent containing either glycogen or linear acrylamide according to the manufacturer's protocol, and then treated with DNaseI (Ambion).
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix IVT labeling protocol. See Affymetrix Expression Analysis Technical Manual
|
| |
|
| Hybridization protocol |
Following fragmentation, ten micrograms of adjusted cRNA fromeach sample was hybridized for 16 hours at 45 deg C to Affymetrix GeneChip
|
| Scan protocol |
Scanning was performed using the Affymetrix GeneChip 3000 Scanner. Images were processed into CEL files with the Affymetrix GCOS software.
|
| Description |
Majeti_32_LSC.CEL
|
| Data processing |
Raw CEL intensity data were normalized using MAS5
|
| |
|
| Submission date |
Sep 07, 2010 |
| Last update date |
Dec 22, 2010 |
| Contact name |
Ash A Alizadeh |
| E-mail(s) |
arasha@stanford.edu
|
| Organization name |
Stanford University School of Medicine
|
| Department |
Medicine, Divisions of Hematology and of Oncology
|
| Lab |
Alizadeh
|
| Street address |
269 Campus Drive, CCSR 1145c
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platform ID |
GPL10881 |
| Series (1) |
| GSE24006 |
A Leukemic Stem Cell Expression Signature is Associated with Clinical Outcomes in Acute Myeloid Leukemia |
|
