NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM5910753 Query DataSets for GSM5910753
Status Public on Feb 02, 2023
Title Y1CTL_RIbo rep2
Sample type SRA
 
Source name inguinal white adipose tissue
Organism Mus musculus
Characteristics strain: C57BL/6
tissue: inguinal white adipose tissue
age: 9-week old
genotype: Y1CTL
Extracted molecule total RNA
Extraction protocol Inguinal adipose tissue was removed and lysis, 1/5 for RNA-seq, 4/5 for Ribosome profiling and further Ribo-seq.
RNA libraries were prepared for sequencing using standard Illumina protocols
 
Library strategy OTHER
Library source transcriptomic
Library selection other
Instrument model Illumina HiSeq 2000
 
Data processing Library strategy: Ribo-seq
Illumina Casava1.7 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm8 whole genome using Hisat2 v2.2.1
Reads Per Kilobase of exon per Megabase of library size (RPKM) were calculated using a protocol from Chepelev et al., Nucleic Acids Research, 2009.
DEseq2
 
Submission date Feb 22, 2022
Last update date Feb 04, 2023
Contact name Sujun Yan
E-mail(s) 291101@zju.edu.cn
Phone +8617326088690
Organization name Zhejiang University
Street address 866 Yuhangtang Rd
City Hangzhou
State/province Zhejiang
ZIP/Postal code 310000
Country China
 
Platform ID GPL13112
Series (1)
GSE197173 Adipocyte YTH N(6)-methyladenosine RNA-binding protein 1 protects against obesity by promoting white adipose tissue beiging in male mice
Relations
BioSample SAMN26172252
SRA SRX14249906

Supplementary file Size Download File type/resource
GSM5910753_rpkm_Y1WT2_Ribo.txt.gz 404.8 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap