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Status |
Public on Feb 02, 2023 |
Title |
Y1cKO_RNA rep2 |
Sample type |
SRA |
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Source name |
inguinal white adipose tissue
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Organism |
Mus musculus |
Characteristics |
strain: C57BL/6 tissue: inguinal white adipose tissue age: 9-week old genotype: Y1cKO
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Extracted molecule |
total RNA |
Extraction protocol |
Inguinal adipose tissue was removed and lysis, 1/5 for RNA-seq, 4/5 for Ribosome profiling and further Ribo-seq. RNA libraries were prepared for sequencing using standard Illumina protocols
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Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2000 |
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Data processing |
Illumina Casava1.7 software used for basecalling. Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm8 whole genome using Hisat2 v2.2.1 Reads Per Kilobase of exon per Megabase of library size (RPKM) were calculated using a protocol from Chepelev et al., Nucleic Acids Research, 2009. DEseq2
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Submission date |
Feb 22, 2022 |
Last update date |
Feb 04, 2023 |
Contact name |
Sujun Yan |
E-mail(s) |
291101@zju.edu.cn
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Phone |
+8617326088690
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Organization name |
Zhejiang University
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Street address |
866 Yuhangtang Rd
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City |
Hangzhou |
State/province |
Zhejiang |
ZIP/Postal code |
310000 |
Country |
China |
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Platform ID |
GPL13112 |
Series (1) |
GSE197173 |
Adipocyte YTH N(6)-methyladenosine RNA-binding protein 1 protects against obesity by promoting white adipose tissue beiging in male mice |
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Relations |
BioSample |
SAMN26172254 |
SRA |
SRX14249904 |
Supplementary file |
Size |
Download |
File type/resource |
GSM5910751_rpkm_Y1CKO2_RNA.txt.gz |
419.1 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
Raw data are available in SRA |
Processed data provided as supplementary file |
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