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Sample GSM5910751 Query DataSets for GSM5910751
Status Public on Feb 02, 2023
Title Y1cKO_RNA rep2
Sample type SRA
 
Source name inguinal white adipose tissue
Organism Mus musculus
Characteristics strain: C57BL/6
tissue: inguinal white adipose tissue
age: 9-week old
genotype: Y1cKO
Extracted molecule total RNA
Extraction protocol Inguinal adipose tissue was removed and lysis, 1/5 for RNA-seq, 4/5 for Ribosome profiling and further Ribo-seq.
RNA libraries were prepared for sequencing using standard Illumina protocols
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
 
Data processing Illumina Casava1.7 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm8 whole genome using Hisat2 v2.2.1
Reads Per Kilobase of exon per Megabase of library size (RPKM) were calculated using a protocol from Chepelev et al., Nucleic Acids Research, 2009.
DEseq2
 
Submission date Feb 22, 2022
Last update date Feb 04, 2023
Contact name Sujun Yan
E-mail(s) 291101@zju.edu.cn
Phone +8617326088690
Organization name Zhejiang University
Street address 866 Yuhangtang Rd
City Hangzhou
State/province Zhejiang
ZIP/Postal code 310000
Country China
 
Platform ID GPL13112
Series (1)
GSE197173 Adipocyte YTH N(6)-methyladenosine RNA-binding protein 1 protects against obesity by promoting white adipose tissue beiging in male mice
Relations
BioSample SAMN26172254
SRA SRX14249904

Supplementary file Size Download File type/resource
GSM5910751_rpkm_Y1CKO2_RNA.txt.gz 419.1 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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