|
| Status |
Public on Feb 22, 2022 |
| Title |
S06_20X |
| Sample type |
RNA |
| |
|
| Source name |
BAL Fluid_TDMT422_total RNA_myeloid-derived suppressor cell
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: BAL Fluid
pre_alveolar macrophageplification: 20X
patient condition: Tuberculosis
cell type: myeloid-derived suppressor cell
patient_id: TDMT422
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Pre-alveolar macrophageplification protocol: cDNA were further subjected to either 16 or 20 pre-alveolar macrophageplification cycles using Fluidigm One Tube Prealveolar macrophagep Master Mix (Fluidigm PN 100-5580)
RNEasy® Micro Kit (Qiagen, Germany)
|
| Label |
N/A
|
| Label protocol |
N/A
|
| |
|
| Hybridization protocol |
N/A
|
| Scan protocol |
N/A
|
| Description |
S06_20X
|
| Data processing |
import into R and normalisation based on 3 reference genes using BioConductor packages readqPCR and normqPCR
statistical analysis using Stan via the brms package
|
| |
|
| Submission date |
Feb 21, 2022 |
| Last update date |
Feb 22, 2022 |
| Contact name |
Gian van der Spuy |
| Organization name |
Stellenbosch University
|
| Department |
Biomedical Sciences
|
| Street address |
Francie van Zijl Dr
|
| City |
Parow |
| ZIP/Postal code |
7530 |
| Country |
South Africa |
| |
|
| Platform ID |
GPL31965 |
| Series (1) |
| GSE197113 |
Targeted gene expression profiling of human myeloid cells from blood and lung compartments of patients with Tuberculosis and other lung diseases |
|
