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Sample GSM5898957 Query DataSets for GSM5898957
Status Public on Jun 12, 2023
Title Fetal Testis 1 [FT1]
Sample type SRA
Source name Fetal_Testis
Organism Homo sapiens
Characteristics tissue type: Fetal_Testis
batch: 144
Extracted molecule total RNA
Extraction protocol RNA was isolated from frozen tissue samples using the total RNA Purification Kit (Norgen Biotek)
RNA was reversed transcribed using Superscript VILO cDNA synthesis kit (Thermo Fisher Scientific) using a C1000 Thermal cycler (Biorad)
Libraries were constructed using the Ion AmpliSeq Transcriptome Human Gene Expression kit (Thermofisher)
Templating and (RNA) sequencing were performed with the Ion PI HiQ OT200 kit, and Ion PI HiQ sequencing kit using Ion PI V3 chips (Life Technologies) and a Proton Sequencer (Thermofisher).
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Ion Torrent Proton
Description barcode: IonXpress_012
Data processing Mapping of read data was performing automatically on the Torrent Suite server, against a reference fasta file composed of the transcript sequences of the target genes, and total read counts per target gene were then tallied.
Submission date Feb 14, 2022
Last update date Jun 12, 2023
Contact name Mariska AM Schröder
Organization name Radboud university medical center
Department Department of Pediatric Endocrinology and Department of Laboratory Medicine
Street address Geert Grooteplein
City Nijmegen
ZIP/Postal code 6500HB
Country Netherlands
Platform ID GPL17303
Series (1)
GSE196700 Transcriptional comparison of Testicular Adrenal Rest Tumors with fetal and adult tissues
BioSample SAMN25937702
SRA SRX14171047

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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