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Sample GSM5884368 Query DataSets for GSM5884368
Status Public on Jan 25, 2024
Title LAN207A6
Sample type SRA
 
Source name Heart
Organism Mus musculus
Characteristics developmental stage: E13.5
genotype: Dp1TybDyrk1a+/+/-
Extracted molecule total RNA
Extraction protocol Hearts were dissected and dissociated into single cell suspensions using the Neonatal Heart Dissociation kit (Miltenyi Biotech) and gentleMACS Octo Dissociator (Miltenyi Biotech) with gentle MACS C tubes and were subsequently methanol-fixed and stored at -80C.
libraries were prepared with the 10x Chromium 3’ mRNA-Seq kit version 2. The cells were QC’d with trypan blue viability dye and quantified using the EVE automated cell counter. Approximately 2000 – 3000 cells were loaded onto the 10x and prepared according to manufacturer’s instructions.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 4000
 
Description rescue
Data processing Cellranger-2.1.1 was used to deconvolve, align and quantify the per-cell transcriptomes.
Reads were aligned to mouse mm10-1.2.0 (obtained from 10x genomics)
Genome_build: mouse mm10 (mm10-1.2.0 from 10x Genomics)
Supplementary_files_format_and_content: tar file containing 10x counts matrix, features and barcode data from filtered cell ranger outputs
 
Submission date Feb 09, 2022
Last update date Jan 25, 2024
Contact name Victor Tybulewicz
E-mail(s) Victor.T@crick.ac.uk
Organization name Francis Crick Institute
Street address 1 Midland Road
City London
ZIP/Postal code NW1 1AT
Country United Kingdom
 
Platform ID GPL21103
Series (2)
GSE196446 Dyrk1a dosage effects on the transcriptome of embryonic hearts from the Dp1Tyb mouse model of Down syndrome using scRNAseq
GSE196447 Down Syndrome
Relations
BioSample SAMN25817278
SRA SRX14121476

Supplementary file Size Download File type/resource
GSM5884368_LAN207A6.tar.gz 8.2 Mb (ftp)(http) TAR
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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