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Sample GSM5884321 Query DataSets for GSM5884321
Status Public on Jan 25, 2024
Title LAN757A15
Sample type SRA
Source name Heart
Organism Homo sapiens
Characteristics developmental stage: 13 pcw
Sex: male
number of chromosomes: 47 chromosomes
Extracted molecule total RNA
Extraction protocol samples were obtained and snap frozen and kept at -80C. Samples were lysed (buffer RLT Qiagen Rneasy Maxi kit) and homogenised using Macs Milteni M tubes and gentleMACS dissociator followed by RNA extraction using Qiagen Rneasy Maxi kit following the standard protocol
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 4000
Description DS
Data processing Read trimming and adaptor removal with Trimmomatic (v 0.36)
Read alignment with STAR ( v 2.5.2a) and gene quantification with RSEM( v 1.2.31)
Differential expression analysis DESeq2
Genome_build: GRCh38 Ensembl release 89
Supplementary_files_format_and_content: table of normalized counts per gene per sample obtained from DESeq
Submission date Feb 09, 2022
Last update date Jan 25, 2024
Contact name Victor Tybulewicz
Organization name Francis Crick Institute
Street address 1 Midland Road
City London
ZIP/Postal code NW1 1AT
Country United Kingdom
Platform ID GPL20301
Series (2)
GSE196443 Transcriptomic analysis of human foetal hearts with or without Down Syndrome
GSE196447 Down Syndrome
BioSample SAMN25816052
SRA SRX14121135

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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