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Sample GSM587878 Query DataSets for GSM587878
Status Public on Aug 28, 2010
Title explants injected with ICD, replicate 3
Sample type RNA
 
Source name explants from embryos injected with ICD RNA
Organism Xenopus laevis
Characteristics treatment: injected at 2-cell stage
culture: explanted at stage 9/10
developmental stage: harvested at equivalent of stage 22
agent: ICD
tissue: epithelial explant
Treatment protocol Animal caps were cut, explanted and cultured in Danilchik's + 0.1% BSA from stage 9/10 until harvest at stage 22
Growth protocol Xenopus embryos grown in 0.1X MMR until stage 9/10
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using the Proteinase K method
Label biotin
Label protocol Biotynylated cRNA was prepared according to standard Affymetrix Protocols
 
Hybridization protocol 10ug of cRNA was hybridized to Xenopus laevis Affymetrix chips for 16hrs at 45C, then washed and stained using the Affymetrix Fluidics Station
Scan protocol GeneChip Scanner 3000 7G from Affymetrix
Data processing Data was processed using Microarray Suite 5.0 software using Affymetrix default settings. Statistical analysis was later performed using Bullfrog software
 
Submission date Aug 27, 2010
Last update date Aug 27, 2010
Contact name Ian K Quigley
E-mail(s) iquigley@salk.edu
Phone 858-453-4100
Organization name Salk Institute
Department Molecular Neurobiology Lab
Lab Kintner
Street address 10010 North Torrey Pines Road
City La Jolla
State/province CA
ZIP/Postal code 92037
Country USA
 
Platform ID GPL1318
Series (1)
GSE23844 Analysis of Notch signaling on Xenopus epidermis

Data table header descriptions
ID_REF
VALUE MAS 5.0 signal
ABS_CALL

Data table
ID_REF VALUE ABS_CALL
AFFX-BioB-5_at 214.2 P
AFFX-BioB-M_at 294.3 P
AFFX-BioB-3_at 187.6 P
AFFX-BioC-5_at 404.8 P
AFFX-BioC-3_at 310 P
AFFX-BioDn-5_at 423.6 P
AFFX-BioDn-3_at 2228.3 P
AFFX-CreX-5_at 3621.3 P
AFFX-CreX-3_at 5762.9 P
AFFX-DapX-5_at 10.2 A
AFFX-DapX-M_at 9.4 A
AFFX-DapX-3_at 0.9 A
AFFX-LysX-5_at 5.8 A
AFFX-LysX-M_at 2.4 A
AFFX-LysX-3_at 2.2 A
AFFX-PheX-5_at 3 A
AFFX-PheX-M_at 0.6 A
AFFX-PheX-3_at 3.1 A
AFFX-ThrX-5_at 1.8 A
AFFX-ThrX-M_at 5.5 A

Total number of rows: 15611

Table truncated, full table size 363 Kbytes.




Supplementary file Size Download File type/resource
GSM587878_Xenopus_ICD_3.CEL.gz 2.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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