NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM5862805 Query DataSets for GSM5862805
Status Public on Feb 09, 2022
Title 10hr post-neomycin
Sample type SRA
 
Source name neuromast
Organism Danio rerio
Characteristics age: 5dpf
tissue: neuromast
treatment/time point: 10hr post-neomycin
strain: Tg(myo6b:H2B-mScarlet-I/Et20:GFP)
Extracted molecule total RNA
Extraction protocol Presumptive neuromast cells (GFP+) were isolated by FACS.
Standard 10x genomics scRNA-seq protocol using v2 chemistry
Droplet based scRNA-seq
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
 
Data processing Raw reads were demultiplexed and aligned to version 10 of the zebrafish genome (GRCz10), ensembl release 91, using the Cell Ranger (version 2.1.1) pipeline from 10x genomics using the default settings.
Genome_build: danRer10
Supplementary_files_format_and_content: h5 files contain the UMI counts for each cell.
 
Submission date Feb 06, 2022
Last update date Feb 10, 2022
Contact name Tatjana Piotrowski
E-mail(s) pio@stowers.org
Organization name Stowers Institute for Medical Research
Lab Piotrowski Lab
Street address 1000 E 50th St
City Kansas City
State/province MO
ZIP/Postal code 64110
Country USA
 
Platform ID GPL14875
Series (1)
GSE196211 High-resolution single cell transcriptome analysis of zebrafish sensory hair cell regeneration
Relations
BioSample SAMN25689699
SRA SRX14059608

Supplementary file Size Download File type/resource
GSM5862805_10hr_filtered_feature_bc_matrix.h5 6.3 Mb (ftp)(http) H5
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap