|
| Status |
Public on Feb 09, 2022 |
| Title |
30min post-neomycin |
| Sample type |
SRA |
| |
|
| Source name |
neuromast
|
| Organism |
Danio rerio |
| Characteristics |
age: 5dpf
tissue: neuromast
treatment/time point: 30min post-neomycin
strain: Tg(myo6b:H2B-mScarlet-I/Et20:GFP)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Presumptive neuromast cells (GFP+) were isolated by FACS.
Standard 10x genomics scRNA-seq protocol using v2 chemistry
Droplet based scRNA-seq
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
Raw reads were demultiplexed and aligned to version 10 of the zebrafish genome (GRCz10), ensembl release 91, using the Cell Ranger (version 2.1.1) pipeline from 10x genomics using the default settings.
Genome_build: danRer10
Supplementary_files_format_and_content: h5 files contain the UMI counts for each cell.
|
| |
|
| Submission date |
Feb 06, 2022 |
| Last update date |
Feb 10, 2022 |
| Contact name |
Tatjana Piotrowski |
| E-mail(s) |
pio@stowers.org
|
| Organization name |
Stowers Institute for Medical Research
|
| Lab |
Piotrowski Lab
|
| Street address |
1000 E 50th St
|
| City |
Kansas City |
| State/province |
MO |
| ZIP/Postal code |
64110 |
| Country |
USA |
| |
|
| Platform ID |
GPL14875 |
| Series (1) |
| GSE196211 |
High-resolution single cell transcriptome analysis of zebrafish sensory hair cell regeneration |
|
| Relations |
| BioSample |
SAMN25689703 |
| SRA |
SRX14059604 |
