|
| Status |
Public on Mar 06, 2023 |
| Title |
RNA-seq_DMSO_mut_2 |
| Sample type |
SRA |
| |
|
| Source name |
heart
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: iPS cardiomyocyte
mutation: Q353R
name in supplementary file: RNA-seq_DMSO_mut_2
|
| Treatment protocol |
Lmna Q353R knock-in mice were generated as previously described (Hara, S. et al. Generation of mutant mice via the CRISPR/Cas9 system using FokI-dCas9. Sci. Rep. 5, 1–9 (2015).)
|
| Growth protocol |
All the animal experiments were approved by the University of Tokyo Ethics Committee for Animal Experiments and strictly adhered to the guidelines for animal experiments of the University of Tokyo (Approved Number P17-058). All mice were housed in separate cages at a maximum of 6 mice per cage in a specific-pathogen–free, temperature-controlled vivarium under a 12-h light/dark cycle with ad libitum access to food and water.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from iPSCMs was extracted using TRIzol reagents (Thermo Fisher Scientific)
NEBNext® UltraTM ll Directional RNA Library Prep Kit
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
mapping with Bowtie2 (version 2.3.4.1)
mapping with Bowtie2 (version 2.3.4.1)
Genome_build: hg19 from UCSC
Supplementary_files_format_and_content: bw and csv files were generated with featureCounts (version 2.0.1)
|
| |
|
| Submission date |
Feb 04, 2022 |
| Last update date |
Mar 06, 2023 |
| Contact name |
Shintaro Yamada |
| E-mail(s) |
shintayamada-tky@g.ecc.u-tokyo.ac.jp
|
| Phone |
81338155411
|
| Organization name |
The University of Tokyo
|
| Department |
Department of Cardiovascular Medicine
|
| Street address |
7-3-1, Hongo, Bunkyo-ku
|
| City |
Tokyo |
| ZIP/Postal code |
113-8655 |
| Country |
Japan |
| |
|
| Platform ID |
GPL24676 |
| Series (1) |
| GSE190977 |
TEAD1 trapping by the Q353R-Lamin A/C causes dilated cardiomyopathy. |
|
| Relations |
| BioSample |
SAMN25652930 |
| SRA |
SRX14044758 |
