|
| Status |
Public on Mar 06, 2023 |
| Title |
ChIP-seq_TEAD1_WT |
| Sample type |
SRA |
| |
|
| Source name |
heart
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: iPS cardiomyocyte
mutation: WT
name in supplementary file: TEAD1_WT
|
| Treatment protocol |
Lmna Q353R knock-in mice were generated as previously described (Hara, S. et al. Generation of mutant mice via the CRISPR/Cas9 system using FokI-dCas9. Sci. Rep. 5, 1–9 (2015).)
|
| Growth protocol |
All the animal experiments were approved by the University of Tokyo Ethics Committee for Animal Experiments and strictly adhered to the guidelines for animal experiments of the University of Tokyo (Approved Number P17-058). All mice were housed in separate cages at a maximum of 6 mice per cage in a specific-pathogen–free, temperature-controlled vivarium under a 12-h light/dark cycle with ad libitum access to food and water.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
ChIP-seq samples (whole genome DNA) from 5×10^4 iPSCMs were extracted using the CUT&RUN assay kit (#86652, Cell Signaling Technology).
KAPA HyperPlus Library Preparation Kit（Kapa Biosystems, #KK8510）
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
mapping with Bowtie2 (version 2.3.4.1)
mapping with Bowtie2 (version 2.3.4.1)
Genome_build: hg19 from UCSC
Supplementary_files_format_and_content: bw and csv files were generated with featureCounts (version 2.0.1)
|
| |
|
| Submission date |
Feb 04, 2022 |
| Last update date |
Mar 08, 2023 |
| Contact name |
Shintaro Yamada |
| E-mail(s) |
shintayamada-tky@g.ecc.u-tokyo.ac.jp
|
| Phone |
81338155411
|
| Organization name |
The University of Tokyo
|
| Department |
Department of Cardiovascular Medicine
|
| Street address |
7-3-1, Hongo, Bunkyo-ku
|
| City |
Tokyo |
| ZIP/Postal code |
113-8655 |
| Country |
Japan |
| |
|
| Platform ID |
GPL16791 |
| Series (1) |
| GSE190977 |
TEAD1 trapping by the Q353R-Lamin A/C causes dilated cardiomyopathy. |
|
| Relations |
| BioSample |
SAMN25652943 |
| SRA |
SRX14044745 |
