|
| Status |
Public on Jul 31, 2011 |
| Title |
ssm201 |
| Sample type |
RNA |
| |
|
| Source name |
Male Subject with Carotid Disease
|
| Organism |
Homo sapiens |
| Characteristics |
gender: male
cell type: Monocytes
|
| Growth protocol |
Monocytes were isolated in a two step process. First, peripheral blood mononuclear cell (PBMC) populations were isolated using a modified density gradient centrifugation.using CPT tubes. Next monocytes were isolated using a negative selection system with magnetic beads coupled to CD3, CD7, CD16, CD19, CD56, CD123 and CD235a (Miltenyi - Monocyte Isolation Kit II)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The total RNA from enriched monocytes was extracted from purified cells using mini RNeasy kits from QIAGEN.
|
| Label |
biotin
|
| Label protocol |
Labeled cRNAs were generated using the Illumina TotalPrep RNA Amplification Kit from Ambion (Cat number IL1791) following the manufacturer’s specifications.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
1496482081_H.csv
|
| Data processing |
Data was quantile normalized without background subtraction using Bioconductor packages
|
| |
|
| Submission date |
Aug 23, 2010 |
| Last update date |
Jul 31, 2011 |
| Contact name |
David K Pritchard |
| E-mail(s) |
dpritch@u.washington.edu
|
| Phone |
1-206-616-8786
|
| Organization name |
University of Washington
|
| Department |
Pathology
|
| Lab |
Schwartz
|
| Street address |
815 Mercer Street Rm. 418
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109-4714 |
| Country |
USA |
| |
|
| Platform ID |
GPL2700 |
| Series (1) |
| GSE23746 |
Monocyte gene expression patterns distinguish subjects with and without atherosclerosis. |
|
