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Sample GSM578843 Query DataSets for GSM578843
Status Public on Aug 14, 2010
Title ovarian epithelial cells T80A2-11_with HMGA2_rep 2
Sample type RNA
 
Source name immortalized ovarian surface epithelial cells
Organism Homo sapiens
Characteristics cell line: immortalized ovarian epithelial cell line T80A2-11
transfection: HMGA2
Growth protocol Cells were maintained at 37oC in 5% CO2.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated by miRNA isolation kit from Ambion according to manufacturer’s protocol.
Label Cy5
Label protocol The labeling was accomplished by Amino Allyl MessageAmp II aRNA Amplification Kit (Ambion) according to manufacturer’s protocol. Firstly, cDNA were synthesized by reverse transcription, including first and second strand synthesis. Then the purified cDNA became the template for in vitro transcription with T7 RNA polymerase. In this step, the modified nucleotide, 5-(3-aminoallyl)-UTP (aaUTP) were incorporated into the aRNA which would be subsequently labeled by Cy5 dye.
 
Hybridization protocol The array was hybridized by using OneArray Hybridization Buffer (Phalanx) for 14 to 16h at 420C which was followed by washing two times per manufacturer’s protocol.
Scan protocol The chips were scanned by Axon 4000 scanner (Molecular Devices).
Description None
Data processing Hierarchical clustering the expression profiles was done by software Cluster 3.0. The genes which have different expression with fold>2 were further analyzed by Pathway-Express.
 
Submission date Aug 12, 2010
Last update date Aug 13, 2010
Contact name Jingjing Wu
E-mail(s) wu_jingjing2007@yahoo.com.cn
Phone 847-530-3863
Organization name northwestern university
Department pathology
Lab ward building 3-031
Street address 303 E. Chicago Avenue
City Chicago
State/province IL
ZIP/Postal code 60611
Country USA
 
Platform ID GPL6254
Series (1)
GSE23599 The gene profile analysis of the function of HMGA2 in immortalized human ovarian surface epithelial cell lines.

Data table header descriptions
ID_REF
VALUE Normalized Intensities

Data table
ID_REF VALUE
PH_hs_0000002 143.33
PH_hs_0000003 92.5
PH_hs_0000004 232.25
PH_hs_0000005 93.67
PH_hs_0000006 202.83
PH_hs_0000007 1421.17
PH_hs_0000008 554.5
PH_hs_0000009 194.67
PH_hs_0000010 18468.75
PH_hs_0000011 112
PH_hs_0000012 141.25
PH_hs_0000013 1772.83
PH_hs_0000014 232.42
PH_hs_0000015 113.75
PH_hs_0000016 90.25
PH_hs_0000017 87.83
PH_hs_0000018 179.83
PH_hs_0000019 160.42
PH_hs_0000020 7753.17
PH_hs_0000021 142.42

Total number of rows: 30968

Table truncated, full table size 614 Kbytes.




Supplementary file Size Download File type/resource
GSM578843_H12_0607004690.gpr.gz 2.6 Mb (ftp)(http) GPR
Processed data included within Sample table

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