|
| Status |
Public on Oct 28, 2022 |
| Title |
fRPE 5f d10_em8 |
| Sample type |
SRA |
| |
|
| Source name |
fRPE_derived CiPCs
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: 5f d10
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted using the DNeasy blood and tissue kit (Qiagen).
300ng of DNA was used for library construction using the NEBNext Enzymatic Methyl-Seq (NEB), respectively.
|
| |
|
| Library strategy |
MRE-Seq |
| Library source |
genomic |
| Library selection |
Restriction Digest |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Data processing |
The adaptor sequences and low quality bases were trimmed from the reads using the Trim Galore tool ver. 0.6.5.
Reads were aligned to the mouse genome using the Bismark alignment software.
The percentage of bisulfite converted cytosines (representing unmethylated cytosines) and non-converted cytosines (representing methylated cytosines) was calculated by bismark_methylation_extractor
Genome_build: hg38
Supplementary_files_format_and_content: bedgraph
|
| |
|
| Submission date |
Dec 30, 2021 |
| Last update date |
Oct 28, 2022 |
| Contact name |
Guoping Fan |
| E-mail(s) |
guoping.fan@gmail.com
|
| Organization name |
university of california, los angeles
|
| Department |
Human Genetics
|
| Street address |
695 Charles E Young Drive South
|
| City |
los angeles |
| State/province |
ca |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
|
| Platform ID |
GPL20301 |
| Series (2) |
| GSE192816 |
Direct reprogramming of human fetal retinal pigment epithelial cells into photoreceptor-like cells [Methyl-seq] |
| GSE192882 |
Direct reprogramming of human fetal retinal pigment epithelial cells into photoreceptor-like cells |
|
| Relations |
| BioSample |
SAMN24526412 |
| SRA |
SRX13559077 |
