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Sample GSM5731351 Query DataSets for GSM5731351
Status Public on May 01, 2024
Title PTA4
Sample type SRA
 
Source name Parathyroid
Organism Homo sapiens
Characteristics tissue: Parathyroid
gender: male
age: 53
disease state: adenoma 
Treatment protocol Parathyroid adenoma resection
Growth protocol A group of clinical symptoms resulting from excess parathyroid hormone secretion due to primary lesions of parathyroid tissue, including hypercalcemia, increased renal calcium reabsorption and urinary phosphorus excretion, kidney stones, renal calcinosis, and increased resorption of cortical bone
Extracted molecule total RNA
Extraction protocol the cells were resuspended in calcium- and magnesium-free PBS containing 0.04% BSA, followed by centrifugation at 300g for 5 min. The cells were then resuspended in 1mL of ice-cold red blood cell lysis buffer.and incubated at 4 °C for 10 min, followed by the addition of 10 ml of ice-cold PBS and centrifugation at 300g for 10 min. After resuspension, the sample was filtered with a 40-μm cell strainer. Cell viability was then determined using Trypan Blue staining with an automated cell counter.
RNA libraries were prepared for sequencing using standard Illumina protocols
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 4000
 
Data processing cell ranger(version 5.0.0; 10 × Genomics, Pleasanton, CA) was used to process the raw data
Genome_build: hg38
Supplementary_files_format_and_content: Matrix table with raw gene counts for every gene and every cell
 
Submission date Dec 13, 2021
Last update date May 01, 2024
Contact name Ting La
E-mail(s) ting.la@uon.edu.au
Phone 49217970
Organization name UON
Street address University Drive
City Callaghan
State/province NSW
ZIP/Postal code 2308
Country Australia
 
Platform ID GPL20301
Series (1)
GSE190773 KMT2A-driven cyclin D2 upregulation and chronic inflammation as potential drivers of sporadic parathyroid adenoma pathogenesis
Relations
BioSample SAMN23992071
SRA SRX13395805

Supplementary file Size Download File type/resource
GSM5731351_PTA4.txt.gz 23.2 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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