|
|
GEO help: Mouse over screen elements for information. |
|
Status |
Public on May 01, 2024 |
Title |
PTA4 |
Sample type |
SRA |
|
|
Source name |
Parathyroid
|
Organism |
Homo sapiens |
Characteristics |
tissue: Parathyroid gender: male age: 53 disease state: adenoma
|
Treatment protocol |
Parathyroid adenoma resection
|
Growth protocol |
A group of clinical symptoms resulting from excess parathyroid hormone secretion due to primary lesions of parathyroid tissue, including hypercalcemia, increased renal calcium reabsorption and urinary phosphorus excretion, kidney stones, renal calcinosis, and increased resorption of cortical bone
|
Extracted molecule |
total RNA |
Extraction protocol |
the cells were resuspended in calcium- and magnesium-free PBS containing 0.04% BSA, followed by centrifugation at 300g for 5 min. The cells were then resuspended in 1mL of ice-cold red blood cell lysis buffer.and incubated at 4 °C for 10 min, followed by the addition of 10 ml of ice-cold PBS and centrifugation at 300g for 10 min. After resuspension, the sample was filtered with a 40-μm cell strainer. Cell viability was then determined using Trypan Blue staining with an automated cell counter. RNA libraries were prepared for sequencing using standard Illumina protocols
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 4000 |
|
|
Data processing |
cell ranger(version 5.0.0; 10 × Genomics, Pleasanton, CA) was used to process the raw data Genome_build: hg38 Supplementary_files_format_and_content: Matrix table with raw gene counts for every gene and every cell
|
|
|
Submission date |
Dec 13, 2021 |
Last update date |
May 01, 2024 |
Contact name |
Ting La |
E-mail(s) |
ting.la@uon.edu.au
|
Phone |
49217970
|
Organization name |
UON
|
Street address |
University Drive
|
City |
Callaghan |
State/province |
NSW |
ZIP/Postal code |
2308 |
Country |
Australia |
|
|
Platform ID |
GPL20301 |
Series (1) |
GSE190773 |
KMT2A-driven cyclin D2 upregulation and chronic inflammation as potential drivers of sporadic parathyroid adenoma pathogenesis |
|
Relations |
BioSample |
SAMN23992071 |
SRA |
SRX13395805 |
Supplementary file |
Size |
Download |
File type/resource |
GSM5731351_PTA4.txt.gz |
23.2 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
Raw data are available in SRA |
Processed data provided as supplementary file |
|
|
|
|
|