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Status |
Public on Jun 21, 2022 |
Title |
BM cells, Mx1-Cre Sf3b1-K700E: Inv(3)_rep1 |
Sample type |
SRA |
|
|
Source name |
Lineage- cKit+ bone marrow cells in femora and tibiae
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6 donor genotype: CD45.2(+) Mx1-Cre Sf3b1-K700E: Inv(3) recipient genotype: CD45.1(+) CD45.2(-) timing of preparation: 6 months after pIpC injection tissue: bone marrow
|
Treatment protocol |
Four weeks after BMT, the recipient mice received 20 mg/kg pIpC injection every other day for a total of 3 doses.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted using RNeasy columns (Qiagen) per manufacturer’s instructions. RNA was poly(A)-selected, and unstranded Illumina libraries were prepared with the standard TruSeq protocol
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 4000 |
|
|
Data processing |
bcl2fastq nf-core/rnaseq 3.0 Genome_build: mm10 Supplementary_files_format_and_content: gene expression (counts, TPMs, FPKMs)
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|
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Submission date |
Dec 10, 2021 |
Last update date |
Jun 23, 2022 |
Contact name |
Masaki Nomura |
E-mail(s) |
masaki.nomura.fbri@gmail.com
|
Organization name |
Foundation for Biomedical Research and Innovation at Kobe
|
Street address |
6-3-7 Minatojima Minamimachi, Chuo Ward
|
City |
Kobe |
State/province |
Hyogo |
ZIP/Postal code |
650-0047 |
Country |
Japan |
|
|
Platform ID |
GPL21103 |
Series (2) |
GSE190655 |
Aberrant EVI1 splicing contributes to EVI1-rearranged leukemia [mouse_RNA-seq] |
GSE190656 |
Aberrant EVI1 splicing contributes to EVI1-rearranged leukemia |
|
Relations |
BioSample |
SAMN23887338 |
SRA |
SRX13377211 |