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Sample GSM5727337 Query DataSets for GSM5727337
Status Public on Jun 21, 2022
Title BM cells, Mx1-Cre Inv(3)_rep3
Sample type SRA
 
Source name Lineage- cKit+ bone marrow cells in femora and tibiae
Organism Mus musculus
Characteristics strain: C57BL/6
donor genotype: CD45.2(+) Mx1-Cre Inv(3)
recipient genotype: CD45.1(+) CD45.2(-)
timing of preparation: 6 months after pIpC injection
tissue: bone marrow
Treatment protocol Four weeks after BMT, the recipient mice received 20 mg/kg pIpC injection every other day for a total of 3 doses.
Extracted molecule total RNA
Extraction protocol RNA was extracted using RNeasy columns (Qiagen) per manufacturer’s instructions.
RNA was poly(A)-selected, and unstranded Illumina libraries were prepared with the standard TruSeq protocol
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 4000
 
Data processing bcl2fastq
nf-core/rnaseq 3.0
Genome_build: mm10
Supplementary_files_format_and_content: gene expression (counts, TPMs, FPKMs)
 
Submission date Dec 10, 2021
Last update date Jun 23, 2022
Contact name Masaki Nomura
E-mail(s) masaki.nomura.fbri@gmail.com
Organization name Foundation for Biomedical Research and Innovation at Kobe
Street address 6-3-7 Minatojima Minamimachi, Chuo Ward
City Kobe
State/province Hyogo
ZIP/Postal code 650-0047
Country Japan
 
Platform ID GPL21103
Series (2)
GSE190655 Aberrant EVI1 splicing contributes to EVI1-rearranged leukemia [mouse_RNA-seq]
GSE190656 Aberrant EVI1 splicing contributes to EVI1-rearranged leukemia
Relations
BioSample SAMN23887334
SRA SRX13377207

Supplementary file Size Download File type/resource
GSM5727337_MxEvi1_R3.genes.results.gz 461.9 Kb (ftp)(http) RESULTS
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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