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Sample GSM572586 Query DataSets for GSM572586
Status Public on Nov 30, 2011
Title Lung_control_sensitization_rep3
Sample type RNA
 
Channel 1
Source name rhesus lung biopsy
Organism Macaca mulatta
Characteristics subject id: 33675
disease state: control
tissue: lung
protocol: sensitized to HDM antigen
timepoint: 15 weeks post-sensitization
biological replicate: 3 of 8
Treatment protocol sensitized to HDM antigen
Growth protocol N/A
Extracted molecule total RNA
Extraction protocol micro total RNA isolation from animal tissues protocol (Qiagen, Valencia, CA)
Label Cy5
Label protocol T7 RNA polymerase single round of linear amplification
 
Channel 2
Source name Stratagene Universal Human Reference
Organism Homo sapiens
Characteristics reference: Stratagene Universal Human Reference
Treatment protocol N/A
Growth protocol N/A
Extracted molecule total RNA
Extraction protocol micro total RNA isolation from animal tissues protocol (Qiagen, Valencia, CA)
Label Cy3
Label protocol T7 RNA polymerase single round of linear amplification
 
 
Hybridization protocol 18 hours at 60°C with constant rotation on Agilent WHG
Scan protocol Agilent scanner according to the manufacturer’s protocol
Description Biological replicate 3 of 8. Lung biopsy at sensitization from control-treated subject.
Data processing the data were extracted and normalized according to the default settings of Agilent Feature Extraction 7.5. LOWESS normalization, background subtraction, and ratio computation.
 
Submission date Jul 29, 2010
Last update date Nov 30, 2011
Contact name Alex Abbas
E-mail(s) abbas@gene.com
Organization name Genentech, Inc.
Department Bioinformatics
Street address 1 DNA Way
City South San Francisco
State/province CA
ZIP/Postal code 94080
Country USA
 
Platform ID GPL1708
Series (1)
GSE23327 Lung biopsies from sensitized or control rhesus macaques

Data table header descriptions
ID_REF
VALUE log2 ratio (test/reference)

Data table
ID_REF VALUE
1 -4.096
2
3 0.221
4 -0.244
5 0.102
6 -0.924
7
8 -0.511
9 0.44
10 0.436
11
12 0.594
13 -1.043
14
15 -0.608
16 1.705
17 0.047
18 1.578
19 0.339
20 -0.226

Total number of rows: 44290

Table truncated, full table size 491 Kbytes.




Supplementary file Size Download File type/resource
GSM572586.txt.gz 14.6 Mb (ftp)(http) TXT
Processed data included within Sample table

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