|
Status |
Public on Nov 30, 2011 |
Title |
Lung_control_sensitization_rep3 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
rhesus lung biopsy
|
Organism |
Macaca mulatta |
Characteristics |
subject id: 33675 disease state: control tissue: lung protocol: sensitized to HDM antigen timepoint: 15 weeks post-sensitization biological replicate: 3 of 8
|
Treatment protocol |
sensitized to HDM antigen
|
Growth protocol |
N/A
|
Extracted molecule |
total RNA |
Extraction protocol |
micro total RNA isolation from animal tissues protocol (Qiagen, Valencia, CA)
|
Label |
Cy5
|
Label protocol |
T7 RNA polymerase single round of linear amplification
|
|
|
Channel 2 |
Source name |
Stratagene Universal Human Reference
|
Organism |
Homo sapiens |
Characteristics |
reference: Stratagene Universal Human Reference
|
Treatment protocol |
N/A
|
Growth protocol |
N/A
|
Extracted molecule |
total RNA |
Extraction protocol |
micro total RNA isolation from animal tissues protocol (Qiagen, Valencia, CA)
|
Label |
Cy3
|
Label protocol |
T7 RNA polymerase single round of linear amplification
|
|
|
|
Hybridization protocol |
18 hours at 60°C with constant rotation on Agilent WHG
|
Scan protocol |
Agilent scanner according to the manufacturer’s protocol
|
Description |
Biological replicate 3 of 8. Lung biopsy at sensitization from control-treated subject.
|
Data processing |
the data were extracted and normalized according to the default settings of Agilent Feature Extraction 7.5. LOWESS normalization, background subtraction, and ratio computation.
|
|
|
Submission date |
Jul 29, 2010 |
Last update date |
Nov 30, 2011 |
Contact name |
Alex Abbas |
E-mail(s) |
abbas@gene.com
|
Organization name |
Genentech, Inc.
|
Department |
Bioinformatics
|
Street address |
1 DNA Way
|
City |
South San Francisco |
State/province |
CA |
ZIP/Postal code |
94080 |
Country |
USA |
|
|
Platform ID |
GPL1708 |
Series (1) |
GSE23327 |
Lung biopsies from sensitized or control rhesus macaques |
|