|
| Status |
Public on Nov 30, 2011 |
| Title |
Lung_disease_baseline_rep2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
rhesus lung biopsy
|
| Organism |
Macaca mulatta |
| Characteristics |
subject id: 32953
disease state: HDM exposure model of asthma
tissue: lung
protocol: mock-sensitized (PBS)
timepoint: baseline
biological replicate: 2 of 9
|
| Treatment protocol |
mock-sensitized (PBS)
|
| Growth protocol |
N/A
|
| Extracted molecule |
total RNA |
| Extraction protocol |
micro total RNA isolation from animal tissues protocol (Qiagen, Valencia, CA)
|
| Label |
Cy5
|
| Label protocol |
T7 RNA polymerase single round of linear amplification
|
| |
|
| Channel 2 |
| Source name |
Stratagene Universal Human Reference
|
| Organism |
Homo sapiens |
| Characteristics |
reference: Stratagene Universal Human Reference
|
| Treatment protocol |
N/A
|
| Growth protocol |
N/A
|
| Extracted molecule |
total RNA |
| Extraction protocol |
micro total RNA isolation from animal tissues protocol (Qiagen, Valencia, CA)
|
| Label |
Cy3
|
| Label protocol |
T7 RNA polymerase single round of linear amplification
|
| |
|
| |
| Hybridization protocol |
18 hours at 60°C with constant rotation on Agilent WHG
|
| Scan protocol |
Agilent scanner according to the manufacturer’s protocol
|
| Description |
Biological replicate 2 of 9. Lung biopsy at baseline from HDM-treated subject.
|
| Data processing |
the data were extracted and normalized according to the default settings of Agilent Feature Extraction 7.5. LOWESS normalization, background subtraction, and ratio computation.
|
| |
|
| Submission date |
Jul 29, 2010 |
| Last update date |
Nov 30, 2011 |
| Contact name |
Alex Abbas |
| E-mail(s) |
abbas@gene.com
|
| Organization name |
Genentech, Inc.
|
| Department |
Bioinformatics
|
| Street address |
1 DNA Way
|
| City |
South San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94080 |
| Country |
USA |
| |
|
| Platform ID |
GPL1708 |
| Series (1) |
| GSE23327 |
Lung biopsies from sensitized or control rhesus macaques |
|
