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| Status |
Public on Feb 08, 2023 |
| Title |
single-cell_TLE_2 |
| Sample type |
SRA |
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| Source name |
Brain
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| Organism |
Homo sapiens |
| Characteristics |
tissue: Brain
diagnosis: TLE patients
molecule subtype: nuclear RNA
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| Extracted molecule |
total RNA |
| Extraction protocol |
Brain were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries.
Single nuclei were captured in the 10×Genomics Chromium Single Cell 3′ Solution, and RNA-seq libraries were prepared following the manufacturer’s protocol (10×Genomics). The libraries were subjected to high-throughput sequencing on an illumina Novaseq6000 platform, and 150-bp paired-end reads were generated.
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
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| Data processing |
Sample demultiplexing, barcode processing, and single cell 3’ gene counting were performed by the Cell Ranger pipelines (v6.0.2,10X Genomics).
Genome_build: GRCh38
Supplementary_files_format_and_content: TSV files with genes and barcode sequences corresponding to row and column indices, respectively.
Supplementary_files_format_and_content: matrix.mtx: Filtered gene-barcode matrix file (demultiplexed)
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| Submission date |
Dec 08, 2021 |
| Last update date |
Feb 08, 2023 |
| Contact name |
Dijun Chen |
| E-mail(s) |
dijunchen@nju.edu.cn
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| Organization name |
Nanjing University
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| Department |
Life Sciences
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| Street address |
Xianlin
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| City |
Nanjing |
| State/province |
Jiangsu |
| ZIP/Postal code |
310028 |
| Country |
China |
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| Platform ID |
GPL24676 |
| Series (2) |
| GSE190452 |
Lipid-accumulated reactive astrocytes promote seizure activity in epilepsy [single-cell RNA-seq] |
| GSE190453 |
Lipid-accumulated reactive astrocytes promote seizure activity in epilepsy |
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| Relations |
| BioSample |
SAMN23802905 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM5724025_TLE_2_barcodes.tsv.gz |
24.6 Kb |
(ftp)(http) |
TSV |
| GSM5724025_TLE_2_features.tsv.gz |
325.6 Kb |
(ftp)(http) |
TSV |
| GSM5724025_TLE_2_matrix.mtx.gz |
36.5 Mb |
(ftp)(http) |
MTX |
| Raw data not provided for this record |
| Processed data provided as supplementary file |
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