|
| Status |
Public on Aug 20, 2023 |
| Title |
Yang-10X-WT |
| Sample type |
SRA |
| |
|
| Source name |
Bone marrow
|
| Organism |
Mus musculus |
| Characteristics |
cell type: LSK bone marrow cells
strain: C57BL/6
genotype: Brd4_WT
|
| Treatment protocol |
BM c-Kit+ and LSK cells from Mx1Cre+ Brd4 flox/fox and WT littermates were purified by sorting 14 days after poly(I:C) injection.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were pooled from two different mice for each genotype
The libraries for each single cell sample were prepared using the Chromium Single Cell Gene Expression 3’ Version 3 Kit (10X Genomics) with the Chromium Controller.
single-cell RNA-seq
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Sequencing results from 10x Genomics were demultiplexed and converted to FASTQ format by cellranger with a parameter of mkfastq
The FASTQ data was aligned to mouse reference genome and quantified using cellranger with default parameters
Genome_build: mm10
Supplementary_files_format_and_content: h5 files of filtered counts for all genes in each cell
|
| |
|
| Submission date |
Nov 29, 2021 |
| Last update date |
Aug 20, 2023 |
| Contact name |
Hui Yang |
| Organization name |
UTHSCSA
|
| Street address |
5114 medical dr.
|
| City |
San Antonio |
| State/province |
Texas |
| ZIP/Postal code |
78229 |
| Country |
USA |
| |
|
| Platform ID |
GPL24247 |
| Series (2) |
| GSE189835 |
Bromodomain member BRD4 is required for hematopoietic stem cell functions (scRNA-Seq) |
| GSE189836 |
Bromodomain member BRD4 is required for hematopoietic stem cell functions |
|
| Relations |
| BioSample |
SAMN23497011 |
| SRA |
SRX13257128 |
