|
| Status |
Public on Sep 06, 2022 |
| Title |
MB49_Batf3-/-_untreated_1 |
| Sample type |
SRA |
| |
|
| Source name |
bladder cancer
|
| Organism |
Mus musculus |
| Characteristics |
tissue: MB49 Subcutaneous tumors
strain: C57BL/6
genotype: Batf3-/-
marker: CD45+ sorted
treatment: untreated
|
| Treatment protocol |
5 mg/kg of the NTPDase inhibitor sodium polyoxotungstate (POM-1/CD39i, purchased from Santa Cruz Biotechnology, Inc., Dallas, Texas, USA) once per day from day 6 to day 22.
|
| Growth protocol |
MB49 Subcutaneous tumors.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
After single cell suspension, CD45+ cells were sorted.
Barcoded scRNA-seq libraries were prepared using the Chromium Single Cell 3’ v3 Reagent Kit.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
| |
|
| Data processing |
Single-cell RNA sequencing data was collected on a Hiseq X Ten Illumina instrument.
Alignment using Cell Ranger (5.0.0) from 10x Genomics.
Genome_build: mm10 (GRCm38)
Supplementary_files_format_and_content: *.tar: Tar archives include barcodes.tsv.gz, features.tsv.gz, and matrix.mtx.gz files.
|
| |
|
| Submission date |
Nov 18, 2021 |
| Last update date |
Sep 06, 2022 |
| Contact name |
Junyi Hu |
| Organization name |
Union Hospital, Tongji Medical College, Huazhong University of Science and Technology
|
| Department |
Urology
|
| Street address |
No. 1277 Liberation Avenue
|
| City |
Wu Han |
| ZIP/Postal code |
430022 |
| Country |
China |
| |
|
| Platform ID |
GPL21273 |
| Series (1) |
| GSE189127 |
Single-Cell Sequencing Reveals the Overall Changes in Tumor Microenvironment Mediated by CD39i |
|
| Relations |
| BioSample |
SAMN23283075 |
| SRA |
SRX13166377 |
