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Status |
Public on Nov 19, 2021 |
Title |
SAMPLE 42: ZFP-TN |
Sample type |
RNA |
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Source name |
liver
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Organism |
Homo sapiens |
Characteristics |
tissue: HCC tissue paired sample: ZFP-PN
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Treatment protocol |
Paired tissue specimens (tumor and adjacent normal tissues) were collected from the patients, immediately frozen in liquid nitrogen, and stored at -80°C until use.
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Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted by Trizol.
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Label |
SYBR Green
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Label protocol |
The cDNAs for circRNA, mRNA, or miRNA measurement were synthesized using random, oligo(dT)18, or stem-loop primers, respectively, using a RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific, Waltham, MA, USA). qRT-PCR was performed in triplicate using Maxima SYBR Green qPCR Master Mix (Thermo Fisher Scientific) on the CFX connect real-time system (Bio-Rad, Hercules, CA, USA).
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Hybridization protocol |
n/a
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Scan protocol |
n/a
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Description |
HCC
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Data processing |
The above 40 pairs of samples were tested in 3 times due to different collection times of the samples,in cluding 10 pairs of samples once time, 17 pairs of samples once time and 13 pairs of samples once time. Experiments from different batches were calibrated using experimental data of samples from the previous batch. Standard curve method was used to analyze the final result. β-actin as the house keeping gene. Three replicates for each sample.
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Submission date |
Nov 17, 2021 |
Last update date |
Nov 19, 2021 |
Contact name |
Yuntao Bing |
E-mail(s) |
bingyuntao@126.com
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Phone |
+86(010)82267338
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Organization name |
Peking University Third Hospital
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Department |
Department of General Surgery
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Street address |
No.49 Huayuan North Road
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City |
Beijing |
ZIP/Postal code |
100191 |
Country |
China |
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Platform ID |
GPL30970 |
Series (1) |
GSE189043 |
circEPS15 overexpression in hepatocellular carcinoma modulates tumor invasion and migration [qRT-PCR validation] |
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Supplementary data files not provided |
Processed data are available on Series record |
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