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Sample GSM5688614 Query DataSets for GSM5688614
Status Public on Feb 04, 2022
Title Cre Control Rep2
Sample type SRA
 
Source name Skeletal muscle stem cells
Organism Mus musculus
Characteristics tissue: Hindlimb muscles
age: 9-week old
treatment: Tamoxifen
Extracted molecule total RNA
Extraction protocol Not provided
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 1500
 
Data processing Raw reads were trimmed using TrimGalore (v0.6.6; http://www.bioinformatics.babraham.ac.uk/projects/trim_galore/) and mapped to the reference genome (GRCm38, PRI) using HISAT2 (v2.2.1) (Kim et al., 2019). The option ‘--trim3 99’ was used to compare reads of different lengths. Expression level was quantified using featureCounts (v2.0.1) (Liao et al., 2014) and annotation (GRCm38.102). The differentially expressed genes were extracted by DESeq2 (Love et al., 2014).
 
Submission date Nov 12, 2021
Last update date Feb 04, 2022
Contact name Hiroshi Sakai
E-mail(s) sakai.hiroshi.wh@ehime-u.ac.jp
Phone +81-89-960-5925
Organization name Ehime University
Department Proteo-Science Center
Lab Division of Integrative Pathophysiology
Street address Shitsukawa
City Toon
State/province Ehime
ZIP/Postal code 791-0295
Country Japan
 
Platform ID GPL18480
Series (2)
GSE169192 Uhrf1 control the proliferation and the differentiation of satellite cells [RNA-Seq]
GSE169193 Uhrf1 control the proliferation and the differentiation of satellite cells
Relations
BioSample SAMN23095040
SRA SRX13122738

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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