|
Status |
Public on Feb 04, 2022 |
Title |
Cre Control Rep1 |
Sample type |
SRA |
|
|
Source name |
Skeletal muscle stem cells
|
Organism |
Mus musculus |
Characteristics |
tissue: Hindlimb muscles age: 9-week old treatment: Tamoxifen
|
Extracted molecule |
total RNA |
Extraction protocol |
Not provided
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 1500 |
|
|
Data processing |
Raw reads were trimmed using TrimGalore (v0.6.6; http://www.bioinformatics.babraham.ac.uk/projects/trim_galore/) and mapped to the reference genome (GRCm38, PRI) using HISAT2 (v2.2.1) (Kim et al., 2019). The option ‘--trim3 99’ was used to compare reads of different lengths. Expression level was quantified using featureCounts (v2.0.1) (Liao et al., 2014) and annotation (GRCm38.102). The differentially expressed genes were extracted by DESeq2 (Love et al., 2014).
|
|
|
Submission date |
Nov 12, 2021 |
Last update date |
Feb 04, 2022 |
Contact name |
Hiroshi Sakai |
E-mail(s) |
sakai.hiroshi.wh@ehime-u.ac.jp
|
Phone |
+81-89-960-5925
|
Organization name |
Ehime University
|
Department |
Proteo-Science Center
|
Lab |
Division of Integrative Pathophysiology
|
Street address |
Shitsukawa
|
City |
Toon |
State/province |
Ehime |
ZIP/Postal code |
791-0295 |
Country |
Japan |
|
|
Platform ID |
GPL18480 |
Series (2) |
GSE169192 |
Uhrf1 control the proliferation and the differentiation of satellite cells [RNA-Seq] |
GSE169193 |
Uhrf1 control the proliferation and the differentiation of satellite cells |
|
Relations |
BioSample |
SAMN23095039 |
SRA |
SRX13122737 |