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Sample GSM5677784 Query DataSets for GSM5677784
Status Public on Nov 20, 2021
Title Control 2
Sample type RNA
 
Source name Plasma
Organism Homo sapiens
Characteristics disease state: healthy
patient: M02
Extracted molecule total RNA
Extraction protocol Isolation using Plasma/Serum Circulating and Exosomal RNA Purification Kit (Slurry Format, #51000, Norgen Biotek, Canada)
Label fluorescence
Label protocol 1000 attomol of spike-in-miRNA-Oligos osa-miR414 and cel-miR-254
 
Hybridization protocol total miRNA isolated from patient plasma was analyzed by the human v3 miRNA expression assay kit code set (Nanostring Tech.)
Scan protocol n.a.
Description total miRNA isolated from patient plasma was analyzed by the human v3 miRNA expression assay kit code set (Nanostring Tech.)
Data processing Background subtraction and quality control for 800 miRNAs tested in each sample were performed using the nSolver Software (Nanostring Tech., USA). Low abundant miRNAs with expression levels equivalent to the expression rates of negative controls were excluded from downstream analysis. All samples were normalized using the geometric mean of the two spike-in miRNA Oligos cel-miR-254 and osa-miR-414, and three positive ligation controls provided in the Code set.
 
Submission date Nov 05, 2021
Last update date Nov 20, 2021
Contact name Tilo Thottakara
Organization name Universität Hamburg
Department Kardiologie
Lab AG Patten
Street address Martinistr 52
City Hamburg
ZIP/Postal code 20246
Country Germany
 
Platform ID GPL30934
Series (1)
GSE188324 A novel miRNA screen identifies miRNA-4454 as a candidate biomarker for ventricular fibrosis in patients with hypertrophic cardiomyopathy

Data table header descriptions
ID_REF
VALUE normalized counts

Data table
ID_REF VALUE
hsa-let-7a-5p 1
hsa-let-7b-5p 42.33
hsa-let-7c-5p 17.86
hsa-let-7d-5p 17.86
hsa-let-7e-5p 16.53
hsa-let-7f-5p 27.12
hsa-let-7g-5p 33.07
hsa-let-7i-5p 37.04
hsa-miR-1-3p 11.24
hsa-miR-1-5p 31.08
hsa-miR-100-5p 35.05
hsa-miR-101-3p 9.92
hsa-miR-103a-3p 23.81
hsa-miR-105-5p 22.49
hsa-miR-106a-5p,miR-17-5p 17.19
hsa-miR-106b-5p 23.81
hsa-miR-107 48.94
hsa-miR-10a-5p 33.73
hsa-miR-10b-5p 27.78
hsa-miR-1178-3p 25.13

Total number of rows: 798

Table truncated, full table size 16 Kbytes.




Supplementary file Size Download File type/resource
GSM5677784_20170609_30101830870717-01_M02_02.RCC.gz 7.3 Kb (ftp)(http) RCC
Processed data included within Sample table

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