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Sample GSM5631802 Query DataSets for GSM5631802
Status Public on Oct 21, 2021
Title rhebCKO2
Sample type SRA
 
Source name BMSCs
Organism Mus musculus
Characteristics strain: C57BL/6J
genotype: rheb knockout in brown adipocytes
age: 1-month
Sex: male
Growth protocol Mice lacking Rheb in the brown adipocyte lineage were generated by mating RhebFL/FL mice with UCP1-Cre mice.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted with Trizol.
Following purification, the mRNA was fragmented into small pieces using divalent cations under elevated temperature. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the TruSeq RNA Sample Preparation v.2 (cat. no. RS-122-2001, RS-122-2002) (Illumina)
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2500
 
Data processing Total RNA quantity and purity were analyzed using a Bioanalyzer 2100 and an RNA 6000 Nano LabChip kit (Agilent), with RNA integrity values of >7.0.
The final cDNA library were created in accordance with the protocol for the TruSeq RNA Sample Preparation v.2
Before assembly, low-quality reads were removed using Trimmomatic software.
Sequencing reads were aligned to the reference genome using the HISAT2 package.
The mapped reads of each sample were assembled and counted using the featureCounts package and a matrix of read counts was generated.
Genome_build: mm10
Supplementary_files_format_and_content: read counts matrix
 
Submission date Oct 19, 2021
Last update date Oct 21, 2021
Contact name zhipeng zou
E-mail(s) zzp@smu.edu.cn
Organization name southern medical university
Department Cell Biology
Street address tonghe road
City guangzhou
State/province guangdong
ZIP/Postal code 510515
Country China
 
Platform ID GPL17021
Series (2)
GSE186092 genes differentially expressed in bone marrow mesenchymal stem cells (BMSCs) between the control mice (rhebFL/FL) and rheb BAD KO mice (Ucp1-cre; rhebFL/FL)
GSE186094 genes differentially expressed between the control mice (rhebFL/FL) and rheb BAD KO mice (Ucp1-cre; rhebFL/FL)
Relations
BioSample SAMN22380173
SRA SRX12681211

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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