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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Oct 19, 2021 |
| Title |
Liver, Ad-Inpolin-2 |
| Sample type |
SRA |
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| Source name |
Liver, Ad-Inpolin
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| Organism |
Mus musculus |
| Characteristics |
tissue: liver
strain: ob/ob
treatment: Ad-Inpolin
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| Treatment protocol |
The mice were administered with Ad-Inpolin or Ad-GFP (1×109 plaque-forming units) through the tail vein and sacrificed at 12th day of administration.
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| Growth protocol |
Male ob/ob mice aged 8-12 weeks were purchased from GemPharmatech Company (Nanjing, China).
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| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction protocol
library construction protocol
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
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| Data processing |
Paired-end reads were harvested from Illumina NovaSeq 6000 sequencer, and were quality controlled by Q30.
After 3’ adaptor-trimming and low quality reads removing by cutadapt software (v1.9.3), the high quality clean reads were aligned to the mouse reference genome (UCSC MM10) with hisat2 software (v2.0.4).
Then, guided by the Ensembl gtf gene annotation file, cuffdiff software (part of cufflinks) was used to get the gene level FPKM as the expression profiles of mRNA.
Fold change>=2.0, that is, log(FC)>=1.0, p-value<=0.05, and FPKM value>=0.1 in at least one (or a group of) samples as the threshold for differential screening.
Genome_build: MM10
Supplementary_files_format_and_content: mRNA Expression Profiling.xls (FPKM Values)
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| Submission date |
Oct 16, 2021 |
| Last update date |
Oct 19, 2021 |
| Contact name |
Yunchen Luo |
| E-mail(s) |
luoyunchen@sjtu.edu.cn
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| Organization name |
Shanghai Jiao Tong University School of Medicine
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| Street address |
shanghai hongkou
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| City |
Shanghai |
| ZIP/Postal code |
200085 |
| Country |
China |
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| Platform ID |
GPL24247 |
| Series (1) |
| GSE186024 |
Orosomucoid 2 maintains hepatic lipid homeostasis through suppression of de novo lipogenesis |
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| Relations |
| BioSample |
SAMN22346287 |
| SRA |
SRX12645681 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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