|
| Status |
Public on Oct 14, 2022 |
| Title |
mm |
| Sample type |
SRA |
| |
|
| Source name |
bone marrow
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
age: P10
subset: GFP+ Lineage-
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The Bone marrow cell samples were harvested from tibia and femur of P10 interspecies or intraspecies. The samples were dissociated into single cell suspension. The GFP+ Lineage- (561317, BD) cells were sorted by Flow cytometry for library preparation.
The libraries were prepared for sequencing using standard 10X 3'v3 protocols
10x 3'v3 single cell RNAseq
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
The standard celllranger mkfastq software version 3.0 used for basecalling.
The standard celllranger count software version 3.0 used to produced raw expression matrix.
The matrix were normalized and scaled by Seurat v4.0 in R4.0.0 environment.
Genome_build: mm10
Supplementary_files_format_and_content: Matrix
|
| |
|
| Submission date |
Sep 28, 2021 |
| Last update date |
Oct 14, 2022 |
| Contact name |
Guolun Wang |
| Organization name |
Cincinnati Children's Hospital Medical Center
|
| Street address |
3333 Burnet Avenue
|
| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45229 |
| Country |
USA |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE184940 |
The integrated single cell RNAseq analysis of bone marrow cells produced by mouse-mouse intraspecies blastocyst complementation and mouse-rat interspecies blastocyst complementation |
|
| Relations |
| SRA |
SRX12387418 |
