|
| Status |
Public on Mar 02, 2011 |
| Title |
Nkx2-5 [ChIP-seq] |
| Sample type |
SRA |
| |
|
| Source name |
HL-1 cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
cell line: HL-1 cardiac muscle cell line
protocol: Nkx2-5 fbio ChIP
chip antibody: none
|
| Treatment protocol |
Confluent HL-1 cells were infected with adenovirus expressing cardiac troponin T promoter driving rtTA and BirA expression, and TRE promoter driving transcription factor Nkx2-5 for 48 hrs before cells harvest for chromatin preparation
|
| Growth protocol |
HL-1 cells were cultured routinely, and split 1:3 every 3-4 days until full confluence.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Libraries were generated according to Illumina single-end ChIP-seq library preparation ( Cat# IP-102-1001)
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina Genome Analyzer |
| |
|
| Description |
Nkx2-5 fbio ChIP
|
| Data processing |
Alignments was done against build mm9 using bowtie, and peaks detection by Sole-Search ( http://havoc.genomecenter.ucdavis.edu/cgi-bin/chipseq.cgi ). Sole-Search Analysis Summary is available as Series GSE21529 supplementary file.
|
| |
|
| Submission date |
Jun 22, 2010 |
| Last update date |
May 15, 2019 |
| Contact name |
Sek Won Kong |
| E-mail(s) |
swkong@enders.tch.harvard.edu
|
| Phone |
617-919-2689
|
| Organization name |
Boston Children's Hospital
|
| Department |
Informatics Program
|
| Lab |
EN137
|
| Street address |
300 Longwood Avenue
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL9185 |
| Series (1) |
| GSE21529 |
Cardiac transcription factors in HL-1 cells: gene expression and genome binding profiling |
|
| Relations |
| SRA |
SRX022481 |
| BioSample |
SAMN00016515 |
