|
| Status |
Public on Sep 06, 2022 |
| Title |
Day4_B_mock_rep2_RNA |
| Sample type |
SRA |
| |
|
| Source name |
Spleen
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
cell type: B cell
treatment: 40LB+IL4
|
| Treatment protocol |
Cells were treated with vehicle or with 100 ug/mL L-ascorbic acid 2-phosphate at the beginning of culture
|
| Growth protocol |
Naïve B cells were co-cultured with irradiated 40LB stromal cells in the presence of recombinant mouse interleukin-4 (1ng/mL; Peprotech) in complete media (RPMI1640, 10% fetal bovine serum, 2mM GlutaMax, 1x non-essential amino acid, 1mM sodium pyruvate, 10mM HEPES, 50 ug/mL gentamicin, and 55 uM 2-mercaptoethanol). Cells were incubated at 37°C with 5% CO2 for 4 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using Qiagen RNeasy kit. The quality of RNA was assayed using TapeStation (Agilent) to ensure all samples are of high quality (RIN > 9.5)
Libraries were constructed using NEBNext Ultra II Directional RNA Library Prep Kit according to the manufacturer’s protocol and barcoded using the NEBNext Multiplex (unique dual index) Oligos
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
STAR (v 2.7.0)
limma (v3.12)
Genome_build: GRCm38
Supplementary_files_format_and_content: Counts
|
| |
|
| Submission date |
Sep 08, 2021 |
| Last update date |
Sep 06, 2022 |
| Contact name |
Chan-Wang Jerry Lio |
| E-mail(s) |
lio.4@osu.edu
|
| Organization name |
Ohio State University
|
| Street address |
460 W 12th Ave
|
| City |
Columbus |
| State/province |
Ohio |
| ZIP/Postal code |
43210 |
| Country |
USA |
| |
|
| Platform ID |
GPL24247 |
| Series (2) |
| GSE183680 |
Vitamin C Potentiates Plasma Cell Differentiation via TET-mediated DNA modification [RNA-seq] |
| GSE183681 |
Epigenetic Remodeling by Vitamin C Potentiates Plasma Cell Differentiation |
|
| Relations |
| BioSample |
SAMN21185322 |
| SRA |
SRX11989888 |
