|
| Status |
Public on Jun 30, 2022 |
| Title |
mouse E10.5 MPCs KO 1 |
| Sample type |
SRA |
| |
|
| Source name |
pancreatic multipotent progenitor cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
age: embryonic day 10.5
genotype: Ptf1aenh-delta/enh-delta
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Dissections of E10.5 and E13.5 embryonic pancreatic tissue were dissociated to single cells and immediately used to build scRNA or to extract nuclei to build scATAC-Seq libraries.
scRNA-Seq libraries were built using Chromium Single Cell 3′ Library & Gel Bead Kit v3, 10x Genomics) and scATAC-Seq libraries were built using Chromium Single Cell ATAC Library & Gel Bead Kit, 10X Genomics)
OTHER: scRNA-Seq and scATAC-Seq
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
none provided by the submitter
Genome_build: mm10
|
| |
|
| Submission date |
Sep 08, 2021 |
| Last update date |
Jun 30, 2022 |
| Contact name |
Irene Miguel-Escalada |
| E-mail(s) |
irene.miguelescalada@crg.eu
|
| Organization name |
CRG
|
| Street address |
Dr Aiguader 88
|
| City |
BArcelona |
| ZIP/Postal code |
08003 |
| Country |
Spain |
| |
|
| Platform ID |
GPL24247 |
| Series (2) |
| GSE183673 |
Pancreas agenesis mutations disrupt a lead enhancer controlling a developmental enhancer cluster |
| GSE183674 |
Pancreas agenesis mutations disrupt a lead enhancer controlling a developmental enhancer cluster |
|
| Relations |
| BioSample |
SAMN21356636 |
| SRA |
SRX12101869 |
