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Status |
Public on Aug 14, 2024 |
Title |
B3_Ischemia 6 h + Veh_rep3 |
Sample type |
SRA |
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Source name |
Ischemia 6 h + Veh_brain tissue
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Organism |
Mus musculus |
Characteristics |
strain: C57BL/6 tissue: brain age: 8 wks disease state: cerebral ischemia treatment: Vehicle
|
Treatment protocol |
Cerebral ischemic mice were preconditiong treated with lactate or vehicle.
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Extracted molecule |
total RNA |
Extraction protocol |
Brains were removed, flash frozen on dry ice, and total RNA was extracted using TRIzol reagent (Takara, Kyoto, Japan) according to the manufacturer’s instructions cDNA libraries were constructed from 1 μg of total RNA by using a cDNA-PCR Sequencing Kit (SQK-PCS109) according to the protocol.
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Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
PromethION |
|
|
Description |
ONT_BMK210602-AK229-01O0007-01
|
Data processing |
Raw reads were first filtered with minimum average read quality score=7 and minimum read length=500bp. full-length, non-chemiric (FLNC) transcripts were determined by searching for primer at both ends of reads. Clusters of FLNC transcripts were obtained after mapping to reference genome with mimimap2. Differential expression analysis of two conditions/groups was performed using the DESeq R package (1.10.1). Genome_build: GRCm38 Supplementary_files_format_and_content: gene expression of all sample
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Submission date |
Sep 04, 2021 |
Last update date |
Aug 14, 2024 |
Contact name |
Xiao-Yi Xiong |
E-mail(s) |
xiongxy1989@hotmail.com
|
Organization name |
Chengdu University of Traditional Chinese Medicine
|
Street address |
37 Shierqiao Road, 610075, Chengdu, China
|
City |
Chengdu |
ZIP/Postal code |
610075 |
Country |
China |
|
|
Platform ID |
GPL26624 |
Series (1) |
GSE183439 |
RNA Sequencing Analysis of Brain Transcriptomes of Ischemic Stroke Treated With or Without Lactate |
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Relations |
BioSample |
SAMN21221895 |
SRA |
SRX12015943 |