|
| Status |
Public on Oct 07, 2010 |
| Title |
HPC7_Pu1 |
| Sample type |
SRA |
| |
|
| Source name |
HPC-7 Cell Line
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Early haematopoietic cell line derived from ES cells
|
| Treatment protocol |
No treatment beyond normal growth protocol was used.
|
| Growth protocol |
HPC-7 cells were grown in 10% conditioned mSCF and were routinely counted and split every day to be kept at a confluence between 0.7-2 x 106 cells per ml).
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Chromatin was fixed in 0.4% formaldehyde, sheared to 200-500bp using a Bioruptor (Diagenode), immunoprecipitated using the indicated antisera, purified and sequenced using Illumina Genome Analyzers as recommended by the manufacturer.
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina Genome Analyzer II |
| |
|
| Description |
Chromatin IP against Pu.1 protein
|
| Data processing |
Processed alignment files include either .map.txt or .export.txt files. The .map.txt files were created using the Maq software package using the default alignment settings. The .export.txt files were created using the Eland software package, again using the standard settings. All alignments were made to the mm9 mouse genome. Column headings for both file types can be found in the linked Readme file. The .bedgraph files were created using an in house script and show the sequence read denisty across the mm9 mouse genome.
|
| |
|
| Submission date |
Jun 07, 2010 |
| Last update date |
May 15, 2019 |
| Contact name |
Samuel David Foster |
| E-mail(s) |
sdf21@cam.ac.uk
|
| Organization name |
Cambridge Institute for Medical Research
|
| Department |
Department of Haematology
|
| Lab |
Haematopoietic Stem Cell Lab
|
| Street address |
Wellcome Trust/MRC Building, Addenbrooke's Hospital, Hills Road
|
| City |
Cambridge |
| State/province |
Cambridgeshire |
| ZIP/Postal code |
CB2 0XY |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL9250 |
| Series (3) |
| GSE22178 |
Combinatorial Transcriptional Control in Blood Stem/Progenitor Cells: Genome-wide Analysis of 10 major Transcriptional Regulators |
| GSE51337 |
Divergent functions of hematopoietic transcription factors in lineage priming and differentiation during erythro-megakaryopoiesis |
| GSE51338 |
Dynamic shifts in occupancy by TAL1 are guided by GATA factors and drive large-scale reprogramming of gene expression during hematopoiesis |
|
| Relations |
| SRA |
SRX021816 |
| BioSample |
SAMN00014605 |
